A Systematic Quantification of Carbonic Anhydrase Transcripts in the Mouse Digestive System

Overview

Journal BMC Mol Biol

Publisher Biomed Central

Specialty Molecular Biology

Date 2007 Mar 17

PMID 17362527

Citations 12

Authors

Pei-wen Pan

Alejandra Rodriguez

Seppo Parkkila

Affiliations

Soon will be listed here.

Abstract

Background: Carbonic anhydrases (CAs) are physiologically important enzymes which participate in many gastrointestinal processes such as acid and bicarbonate secretion and metabolic pathways including gluconeogenesis and ureagenesis. The genomic data suggests that there are thirteen enzymatically active members of the mammalian CA isozyme family. In the present study, we systematically examined the mRNA expression levels of all known CA isozymes by quantitative real-time PCR in eight tissues of the digestive system of male and female mice.

Results: The CAs expressed in all tissues were Car5b, Car7, and Car15, among which Car5b showed moderate and Car7 and Car15 extremely low expression levels. Car3, Car12, Car13, and Car14 were detected in seven out of eight tissues and Car2 and Car4 were expressed in six tissues. Importantly, Car1, Car3, and Car13 showed very high expression levels in certain tissues as compared to the other CAs, suggesting that these low activity isozymes may also participate in physiological processes other than CA catalysis and high expression levels are required to fulfil their functions in the body.

Conclusion: A comprehensive mRNA expression profile of the 13 enzymatically active CAs in the murine gastrointestinal tract was produced in the present study. It contributes to a deeper understanding of the distribution of CA isozymes and their potential roles in the mouse digestive system.

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References

Pastorekova S, Parkkila S, Parkkila A, Opavsky R, ZelnIk V, Saarnio J . Carbonic anhydrase IX, MN/CA IX: analysis of stomach complementary DNA sequence and expression in human and rat alimentary tracts. Gastroenterology. 1997; 112(2):398-408. DOI: 10.1053/gast.1997.v112.pm9024293. View

Fleming R, Parkkila S, Parkkila A, Rajaniemi H, Waheed A, Sly W . Carbonic anhydrase IV expression in rat and human gastrointestinal tract regional, cellular, and subcellular localization. J Clin Invest. 1995; 96(6):2907-13. PMC: 186002. DOI: 10.1172/JCI118362. View

Saarnio J, Parkkila S, Parkkila A, Waheed A, Casey M, Zhou X . Immunohistochemistry of carbonic anhydrase isozyme IX (MN/CA IX) in human gut reveals polarized expression in the epithelial cells with the highest proliferative capacity. J Histochem Cytochem. 1998; 46(4):497-504. DOI: 10.1177/002215549804600409. View

Tureci O, Sahin U, Vollmar E, Siemer S, Gottert E, Seitz G . Human carbonic anhydrase XII: cDNA cloning, expression, and chromosomal localization of a carbonic anhydrase gene that is overexpressed in some renal cell cancers. Proc Natl Acad Sci U S A. 1998; 95(13):7608-13. PMC: 22698. DOI: 10.1073/pnas.95.13.7608. View

Earnhardt J, Qian M, Tu C, Lakkis M, Bergenhem N, Laipis P . The catalytic properties of murine carbonic anhydrase VII. Biochemistry. 1998; 37(30):10837-45. DOI: 10.1021/bi980046t. View

Nishimori I, Taguchi T, Onishi S . Human mitochondrial carbonic anhydrase VB. cDNA cloning, mRNA expression, subcellular localization, and mapping to chromosome x. J Biol Chem. 1999; 274(30):21228-33. DOI: 10.1074/jbc.274.30.21228. View

Kivela A, Kivela J, Saarnio J, Parkkila S . Carbonic anhydrases in normal gastrointestinal tract and gastrointestinal tumours. World J Gastroenterol. 2005; 11(2):155-63. PMC: 4205394. DOI: 10.3748/wjg.v11.i2.155. View

Purkerson J, Schwartz G . Expression of membrane-associated carbonic anhydrase isoforms IV, IX, XII, and XIV in the rabbit: induction of CA IV and IX during maturation. Am J Physiol Regul Integr Comp Physiol. 2005; 288(5):R1256-63. DOI: 10.1152/ajpregu.00735.2004. View

Kivela A, Parkkila S, Saarnio J, Karttunen T, Kivela J, Parkkila A . Expression of von Hippel-Lindau tumor suppressor and tumor-associated carbonic anhydrases IX and XII in normal and neoplastic colorectal mucosa. World J Gastroenterol. 2005; 11(17):2616-25. PMC: 4305753. DOI: 10.3748/wjg.v11.i17.2616. View

10.

Hilvo M, Tolvanen M, Clark A, Shen B, Shah G, Waheed A . Characterization of CA XV, a new GPI-anchored form of carbonic anhydrase. Biochem J. 2005; 392(Pt 1):83-92. PMC: 1317667. DOI: 10.1042/BJ20051102. View

11.

Shah G, Ulmasov B, Waheed A, Becker T, Makani S, Svichar N . Carbonic anhydrase IV and XIV knockout mice: roles of the respective carbonic anhydrases in buffering the extracellular space in brain. Proc Natl Acad Sci U S A. 2005; 102(46):16771-6. PMC: 1283849. DOI: 10.1073/pnas.0508449102. View

12.

Pan P, Leppilampi M, Pastorekova S, Pastorek J, Waheed A, Sly W . Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice. J Physiol. 2006; 571(Pt 2):319-27. PMC: 1796798. DOI: 10.1113/jphysiol.2005.102590. View

13.

Lenaerts K, Sokolovic M, Bouwman F, Lamers W, Mariman E, Renes J . Starvation induces phase-specific changes in the proteome of mouse small intestine. J Proteome Res. 2006; 5(9):2113-22. DOI: 10.1021/pr060183+. View

14.

Leppilampi M, Parkkila S, Karttunen T, Gut M, Gros G, Sjoblom M . Carbonic anhydrase isozyme-II-deficient mice lack the duodenal bicarbonate secretory response to prostaglandin E2. Proc Natl Acad Sci U S A. 2005; 102(42):15247-52. PMC: 1257747. DOI: 10.1073/pnas.0508007102. View

15.

Parkkila S, Halsted C, Villanueva J, Vaananen H, Niemela O . Expression of testosterone-dependent enzyme, carbonic anhydrase III, and oxidative stress in experimental alcoholic liver disease. Dig Dis Sci. 1999; 44(11):2205-13. DOI: 10.1023/a:1026640317233. View

16.

Shah G, Grubb J, Migas M, Fleming R, Waheed A, Sly W . Mitochondrial carbonic anhydrase CA VB: differences in tissue distribution and pattern of evolution from those of CA VA suggest distinct physiological roles. Proc Natl Acad Sci U S A. 2000; 97(4):1677-82. PMC: 26495. DOI: 10.1073/pnas.97.4.1677. View

17.

Parkkila S, Parkkila A, Rajaniemi H, Shah G, Grubb J, Waheed A . Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. Proc Natl Acad Sci U S A. 2001; 98(4):1918-23. PMC: 29357. DOI: 10.1073/pnas.98.4.1918. View

18.

Parkkila S . An overview of the distribution and function of carbonic anhydrase in mammals. EXS. 2001; (90):79-93. DOI: 10.1007/978-3-0348-8446-4_4. View

19.

Parkkila S . Roles of carbonic anhydrases in the alimentary tract. EXS. 2001; (90):462-74. View

20.

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A . Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002; 3(7):RESEARCH0034. PMC: 126239. DOI: 10.1186/gb-2002-3-7-research0034. View