Method to Enhance Transfection Efficiency of Cell Lines and Placental Fibroblasts

Overview

Journal Placenta

Publisher Elsevier

Specialties Gynecology & Obstetrics
Physiology
Reproductive Medicine

Date 2007 Mar 16

PMID 17355893

Citations 15

Authors

M Zhang

S Guller

Y Huang

Affiliations

Soon will be listed here.

Abstract

We report a method that allows for a 2-9-fold increase in transfection efficiency compared to the standard cationic lipid-based protocol. The method involves a brief incubation of freshly trypsinized cells with transfection complexes, followed by incubation in cell growth medium containing serum and antibiotics. The method is simple, cost-effective, and can be applied to both DNA and siRNA transfections as well as to a variety of cell types including hard-to-transfect human placental fibroblasts.

Citing Articles

Genetic Studies of Leptin Concentrations Implicate Leptin in the Regulation of Early Adiposity.

Yaghootkar H, Zhang Y, Spracklen C, Karaderi T, Huang L, Bradfield J Diabetes. 2020; 69(12):2806-2818.

PMID: 32917775 PMC: 7679778. DOI: 10.2337/db20-0070.

A truncated apoptin protein variant selectively kills cancer cells.

Ruiz-Martinez S, Castro J, Vilanova M, Bruix M, Laurents D, Ribo M Invest New Drugs. 2017; 35(3):260-268.

PMID: 28120180 DOI: 10.1007/s10637-017-0431-6.

Fast and Efficient Transfection of Mouse Embryonic Stem Cells Using Non-Viral Reagents.

Tamm C, Kadekar S, Pijuan-Galito S, Anneren C Stem Cell Rev Rep. 2016; 12(5):584-591.

PMID: 27358240 PMC: 5050252. DOI: 10.1007/s12015-016-9673-5.

Decidual cell regulation of natural killer cell-recruiting chemokines: implications for the pathogenesis and prediction of preeclampsia.

Lockwood C, Huang S, Chen C, Huang Y, Xu J, Faramarzi S Am J Pathol. 2013; 183(3):841-56.

PMID: 23973270 PMC: 3763772. DOI: 10.1016/j.ajpath.2013.05.029.

TNF-α up-regulates protein level and cell surface expression of the leptin receptor by stimulating its export via a PKC-dependent mechanism.

Gan L, Guo K, Cremona M, McGraw T, Leibel R, Zhang Y Endocrinology. 2012; 153(12):5821-33.

PMID: 23070544 PMC: 3512064. DOI: 10.1210/en.2012-1510.