Use and Abuse of Exogenous H2O2 in Studies of Signal Transduction

Overview

Journal Free Radic Biol Med

Specialties Biochemistry
Biology
General Medicine

Date 2007 Mar 14

PMID 17349920

Citations 64

Authors

Henry Jay Forman

Affiliations

Soon will be listed here.

Abstract

The goal of this review is to present a rationale for the use of exogenous H(2)O(2), which has been demonstrated to have both toxicological and physiological signaling roles. Reasons for the use of exogenous application of nontoxic concentrations of H(2)O(2) in model systems and caveats for interpretation of the data obtained will both be presented. Briefly, an argument for the cautious use of the addition of exogenous H(2)O(2) is that, because of the permeability of cell membranes to this neutral small molecule, a concentration that is produced locally and that is necessary for the physiological action can be mimicked. On the other hand, it must be recognized that the addition of an agent or its enzymatic generation in the medium may produce reactions that may not normally occur because the total dose of H(2)O(2) and the concentration of H(2)O(2) in some cellular locations will exceed what is normally achieved even under a pathophysiological state. For this reason, this review will try to provide an unbiased balanced pros- and -cons analysis of this issue.

Citing Articles

FRBM Mini REVIEW: Chemogenetic approaches to probe redox dysregulation in heart failure.

Guo R, Spyropoulos F, Michel T Free Radic Biol Med. 2024; 217:173-178.

PMID: 38565399 PMC: 11221410. DOI: 10.1016/j.freeradbiomed.2024.03.027.

D-Amino acid oxidase-derived chemogenetic oxidative stress: Unraveling the multi-omic responses to in vivo redox stress.

Spyropoulos F, Michel T Curr Opin Chem Biol. 2024; 79:102438.

PMID: 38417321 PMC: 10957096. DOI: 10.1016/j.cbpa.2024.102438.

5-Aminolevulinic acid treatment mitigates pesticide stress in bean seedlings by regulating stress-related gene expression and retrotransposon movements.

Yuksel E, Aydin M, Agar G, Taspinar M Protoplasma. 2024; 261(3):581-592.

PMID: 38191719 PMC: 11021237. DOI: 10.1007/s00709-023-01924-9.

Oxidative Stress, Transfer RNA Metabolism, and Protein Synthesis.

Akiyama Y, Ivanov P Antioxid Redox Signal. 2023; 40(10-12):715-735.

PMID: 37767630 PMC: 11001508. DOI: 10.1089/ars.2022.0206.

CHAC1 as a Novel Contributor of Ferroptosis in Retinal Pigment Epithelial Cells with Oxidative Damage.

Liu Y, Wu D, Fu Q, Hao S, Gu Y, Zhao W Int J Mol Sci. 2023; 24(2).

PMID: 36675091 PMC: 9861460. DOI: 10.3390/ijms24021582.

References

Cai H . Hydrogen peroxide regulation of endothelial function: origins, mechanisms, and consequences. Cardiovasc Res. 2005; 68(1):26-36. DOI: 10.1016/j.cardiores.2005.06.021. View

Liu H, Zhang H, Iles K, Rinna A, Merrill G, Yodoi J . The ADP-stimulated NADPH oxidase activates the ASK-1/MKK4/JNK pathway in alveolar macrophages. Free Radic Res. 2006; 40(8):865-74. PMC: 2713795. DOI: 10.1080/10715760600758514. View

Liu J, Finkel T . Stem cell aging: what bleach can teach. Nat Med. 2006; 12(4):383-4. DOI: 10.1038/nm0406-383. View

Klug D, RABANI J, Fridovich I . A direct demonstration of the catalytic action of superoxide dismutase through the use of pulse radiolysis. J Biol Chem. 1972; 247(15):4839-42. View

Loschen G, Azzi A, Richter C, Flohe L . Superoxide radicals as precursors of mitochondrial hydrogen peroxide. FEBS Lett. 1974; 42(1):68-72. DOI: 10.1016/0014-5793(74)80281-4. View

Forman H, Kennedy J . Role of superoxide radical in mitochondrial dehydrogenase reactions. Biochem Biophys Res Commun. 1974; 60(3):1044-50. DOI: 10.1016/0006-291x(74)90418-5. View

Mukherjee S, Lane R, Lynn W . Endogenous hydrogen peroxide and peroxidative metabolism in adipocytes in response to insulin and sulfhydryl reagents. Biochem Pharmacol. 1978; 27(22):2589-94. DOI: 10.1016/0006-2952(78)90332-5. View

Forman H, Skelton D, Loeb G, Dorio R . Membrane permeability and oxidant induced injury. Basic Life Sci. 1988; 49:523-30. DOI: 10.1007/978-1-4684-5568-7_81. View

Takeda K, Hatai T, Hamazaki T, Nishitoh H, Saitoh M, Ichijo H . Apoptosis signal-regulating kinase 1 (ASK1) induces neuronal differentiation and survival of PC12 cells. J Biol Chem. 2000; 275(13):9805-13. DOI: 10.1074/jbc.275.13.9805. View

10.

Schreck R, Rieber P, Baeuerle P . Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1. EMBO J. 1991; 10(8):2247-58. PMC: 452914. DOI: 10.1002/j.1460-2075.1991.tb07761.x. View

11.

Royall J, Gwin P, Parks D, Freeman B . Responses of vascular endothelial oxidant metabolism to lipopolysaccharide and tumor necrosis factor-alpha. Arch Biochem Biophys. 1992; 294(2):686-94. DOI: 10.1016/0003-9861(92)90742-f. View

12.

Kaul N, Forman H . Activation of NF kappa B by the respiratory burst of macrophages. Free Radic Biol Med. 1996; 21(3):401-5. DOI: 10.1016/0891-5849(96)00178-5. View

13.

Wang X, Diener K, Jannuzzi D, Trollinger D, Tan T, Lichenstein H . Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. J Biol Chem. 1996; 271(49):31607-11. DOI: 10.1074/jbc.271.49.31607. View

14.

Ichijo H, Nishida E, Irie K, Ten Dijke P, Saitoh M, Moriguchi T . Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science. 1997; 275(5296):90-4. DOI: 10.1126/science.275.5296.90. View

15.

Krejsa C, Nadler S, Esselstyn J, Kavanagh T, Ledbetter J, Schieven G . Role of oxidative stress in the action of vanadium phosphotyrosine phosphatase inhibitors. Redox independent activation of NF-kappaB. J Biol Chem. 1997; 272(17):11541-9. DOI: 10.1074/jbc.272.17.11541. View

16.

Denu J, Tanner K . Specific and reversible inactivation of protein tyrosine phosphatases by hydrogen peroxide: evidence for a sulfenic acid intermediate and implications for redox regulation. Biochemistry. 1998; 37(16):5633-42. DOI: 10.1021/bi973035t. View

17.

Barrett W, DeGnore J, Keng Y, Zhang Z, Yim M, Chock P . Roles of superoxide radical anion in signal transduction mediated by reversible regulation of protein-tyrosine phosphatase 1B. J Biol Chem. 1999; 274(49):34543-6. DOI: 10.1074/jbc.274.49.34543. View

18.

Liu H, Nishitoh H, Ichijo H, Kyriakis J . Activation of apoptosis signal-regulating kinase 1 (ASK1) by tumor necrosis factor receptor-associated factor 2 requires prior dissociation of the ASK1 inhibitor thioredoxin. Mol Cell Biol. 2000; 20(6):2198-208. PMC: 110836. DOI: 10.1128/MCB.20.6.2198-2208.2000. View

19.

Antunes F, Cadenas E . Estimation of H2O2 gradients across biomembranes. FEBS Lett. 2000; 475(2):121-6. DOI: 10.1016/s0014-5793(00)01638-0. View

20.

Jakubowski W, Bartosz G . 2,7-dichlorofluorescin oxidation and reactive oxygen species: what does it measure?. Cell Biol Int. 2000; 24(10):757-60. DOI: 10.1006/cbir.2000.0556. View