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Differentiation of Giardia Duodenalis from Other Giardia Spp. by Using Polymerase Chain Reaction and Gene Probes

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1992 Jan 1
PMID 1734070
Citations 27
Authors
M H Mahbubani
A K Bej
M H Perlin
F W Schaefer 3rd
W Jakubowski
R M Atlas
Affiliations
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Abstract

Giardia spp. are waterborne organisms that are the most commonly identified pathogenic intestinal protozoans in the United States. Current detection techniques for Giardia species in water include microscopy and immunofluorescence techniques. Species of the genus Giardia are classified on the basis of taxonomic criteria, such as cell morphology, and on host specificity. We have developed a polymerase chain reaction- and gene probe-based detection system specific for Giardia spp., which can discriminate between the relevant species of the G. duodenalis type pathogenic to humans and other Giardia species that are not human pathogens. This method can detect a single Giardia cyst and is therefore sensitive enough for environmental monitoring.

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References
1.
ANSARI M . [The genus Giardia Kunstler, 1882 (Mastigophora: Octomitidae). I. Synoptic list of known and four new species]. Ann Parasitol Hum Comp. 1952; 27(4):421-79. View
2.
Abbaszadegan M, Gerba C, Rose J . Detection of Giardia cysts with a cDNA probe and applications to water samples. Appl Environ Microbiol. 1991; 57(4):927-31. PMC: 182824. DOI: 10.1128/aem.57.4.927-931.1991. View
3.
Campbell S, van Keulen H, Erlandsen S, SENTURIA J, Jarroll E . Giardia sp.: comparison of electrophoretic karyotypes. Exp Parasitol. 1990; 71(4):470-82. DOI: 10.1016/0014-4894(90)90073-l. View
4.
Erlandsen S, Bemrick W, Wells C, Feely D, Knudson L, Campbell S . Axenic culture and characterization of Giardia ardeae from the great blue heron (Ardea herodias). J Parasitol. 1990; 76(5):717-24. View
5.
Baker D, Holberton D, Marshall J . Sequence of a giardin subunit cDNA from Giardia lamblia. Nucleic Acids Res. 1988; 16(14B):7177. PMC: 338359. DOI: 10.1093/nar/16.14.7177. View