Impact of Competitive Inhibition and Sequence Variation Upon the Sensitivity of Malaria PCR

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2007 Mar 3

PMID 17329455

Citations 21

Authors

Seweryn Bialasiewicz

David M Whiley

Michael D Nissen

Theo P Sloots

Affiliations

Soon will be listed here.

Abstract

Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.

Citing Articles

A novel sensitive hexaplex high-resolution melt assay for identification of five human Plasmodium species plus internal control.

Srisutham S, Rattanakoch P, Kijprasong K, Sugaram R, Kantaratanakul N, Srinulgray T Acta Trop. 2023; 248:107020.

PMID: 37739253 PMC: 10641754. DOI: 10.1016/j.actatropica.2023.107020.

Quadruplex Real-Time TaqMan RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes.

Carossino M, Balasuriya U, Thieulent C, Barrandeguy M, Vissani M, Parreno V Viruses. 2023; 15(8).

PMID: 37631969 PMC: 10459720. DOI: 10.3390/v15081626.

Reply to Deora et al. Multiplexing for spp.? Think Again! Comment on "Bhowmick et al. Dry Post Wintertime Mass Surveillance Unearths a Huge Burden of , and Mixed Infection with , a Threat to Malaria Elimination, in Dhalai, Tripura, India. 2021, ,....

Pal-Bhowmick I, Nirmolia T, Pandey A, Subbarao S, Nath A, Senapati S Pathogens. 2022; 11(8).

PMID: 35894055 PMC: 9332397. DOI: 10.3390/pathogens11080832.

Multiplexing for Plasmodium spp.? Think Again! Comment on Bhowmick et al. Dry Post Wintertime Mass Surveillance Unearths a Huge Burden of P. vivax, and Mixed Infection with P. vivax P. falciparum, a Threat to Malaria Elimination, in Dhalai, Tripura,....

Deora N, Kar S, Sinha A Pathogens. 2022; 11(7).

PMID: 35889983 PMC: 9324187. DOI: 10.3390/pathogens11070737.

Usefulness of Malachite-Green LAMP for Diagnosis of Plasmodium and Five Human Malaria Species in a Nonendemic Setting.

Martin Ramirez A, Lanza Suarez M, Munoz Garcia C, Hisam S, Perez-Ayala A, Rubio J Am J Trop Med Hyg. 2022; .

PMID: 35292597 PMC: 9128691. DOI: 10.4269/ajtmh.21-1151.

References

Skarbinski J, James E, Causer L, Barber A, Mali S, Nguyen-Dinh P . Malaria surveillance--United States, 2004. MMWR Surveill Summ. 2006; 55(4):23-37. View

Rougemont M, Van Saanen M, Sahli R, Hinrikson H, Bille J, Jaton K . Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assays. J Clin Microbiol. 2004; 42(12):5636-43. PMC: 535226. DOI: 10.1128/JCM.42.12.5636-5643.2004. View

Charles D, Hart J, Davis W, Sullivan E, Dowse G, Davis T . Notifications of imported malaria in Western Australia, 1990-2001: incidence, associated factors and chemoprophylaxis. Med J Aust. 2005; 182(4):164-7. DOI: 10.5694/j.1326-5377.2005.tb06646.x. View

Elawad B, Ong E . Retrospective study of malaria cases treated in Newcastle General Hospital between 1990 and 1996. J Travel Med. 1999; 5(4):193-7. DOI: 10.1111/j.1708-8305.1998.tb00506.x. View

Kain K, Harrington M, Tennyson S, Keystone J . Imported malaria: prospective analysis of problems in diagnosis and management. Clin Infect Dis. 1998; 27(1):142-9. DOI: 10.1086/514616. View

Perandin F, Manca N, Calderaro A, Piccolo G, Galati L, Ricci L . Development of a real-time PCR assay for detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosis. J Clin Microbiol. 2004; 42(3):1214-9. PMC: 356834. DOI: 10.1128/JCM.42.3.1214-1219.2004. View

McNamara D, Thomson J, Kasehagen L, Zimmerman P . Development of a multiplex PCR-ligase detection reaction assay for diagnosis of infection by the four parasite species causing malaria in humans. J Clin Microbiol. 2004; 42(6):2403-10. PMC: 427836. DOI: 10.1128/JCM.42.6.2403-2410.2004. View

Benito A, Rubio J . Usefulness of seminested polymerase chain reaction for screening blood donors at risk for malaria in Spain. Emerg Infect Dis. 2001; 7(6):1068. PMC: 2631925. DOI: 10.3201/eid0706.010632. View

Whiley D, LeCornec G, Baddeley A, Savill J, Syrmis M, Mackay I . Detection and differentiation of Plasmodium species by polymerase chain reaction and colorimetric detection in blood samples of patients with suspected malaria. Diagn Microbiol Infect Dis. 2004; 49(1):25-9. DOI: 10.1016/j.diagmicrobio.2003.10.014. View

10.

Snounou G, Viriyakosol S, Zhu X, Jarra W, Pinheiro L, do Rosario V . High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol. 1993; 61(2):315-20. DOI: 10.1016/0166-6851(93)90077-b. View

11.

Robinson P, Jenney A, Tachado M, Yung A, Manitta J, Taylor K . Imported malaria treated in Melbourne, Australia: epidemiology and clinical features in 246 patients. J Travel Med. 2001; 8(2):76-81. DOI: 10.2310/7060.2001.24309. View

12.

Whiley D, Sloots T . Melting curve analysis using hybridisation probes: limitations in microbial molecular diagnostics. Pathology. 2005; 37(3):254-6. DOI: 10.1080/00313020500099270. View

13.

Mueller I, Bjorge S, Poigeno G, Kundi J, Tandrapah T, Riley I . The epidemiology of malaria in the Papua New Guinea highlands: 2. Eastern Highlands Province. P N G Med J. 2006; 46(3-4):166-79. View