» Articles » PMID: 17325680

Nuclear Reprogramming of Cloned Embryos and Its Implications for Therapeutic Cloning

Overview
Journal Nat Genet
Specialty Genetics
Date 2007 Feb 28
PMID 17325680
Citations 175
Authors
Affiliations
Soon will be listed here.
Abstract

Therapeutic cloning, whereby somatic cell nuclear transfer (SCNT) is used to generate patient-specific embryonic stem cells (ESCs) from blastocysts cloned by nuclear transfer (ntESCs), holds great promise for the treatment of many human diseases. ntESCs have been derived in mice and cattle, but thus far there are no credible reports of human ntESCs. Here we review the recent literature on nuclear reprogramming by SCNT, including studies of gene expression, DNA methylation, chromatin remodeling, genomic imprinting and X chromosome inactivation. Reprogramming of genes expressed in the inner cell mass, from which ntESCs are derived, seems to be highly efficient. Defects in the extraembryonic lineage are probably the major cause of the low success rate of reproductive cloning but are not expected to affect the derivation of ntESCs. We remain optimistic that human therapeutic cloning is achievable and that the derivation of patient-specific ntESC lines will have great potential for regenerative medicine.

Citing Articles

Transcription coactivator YAP1 promotes CCND1/CDK6 expression, stimulating cell proliferation in cloned cattle placentas.

Wu S, Zhao X, Yang L, Hai C, Wu D, Liu X Zool Res. 2025; 46(1):122-138.

PMID: 39846191 PMC: 11890997. DOI: 10.24272/j.issn.2095-8137.2024.211.


40 years of AETE: the contribution of scientists and practitioners to the progress of reproductive biotechnologies in Europe.

Galli C, Lazzari G Anim Reprod. 2024; 21(3):e20240061.

PMID: 39286367 PMC: 11404877. DOI: 10.1590/1984-3143-AR2024-0061.


Lysophosphatidic acid improves development of porcine somatic cell nuclear transfer embryos.

Sun L, Lin T, Lee J, Kim S, Bai Y, Jin D J Anim Sci Technol. 2024; 66(4):726-739.

PMID: 39165747 PMC: 11331365. DOI: 10.5187/jast.2024.e68.


The Reproductive Lifespan of Ovarian Follicle.

Kalinderi K, Kalinderis M, Papaliagkas V, Fidani L Reprod Sci. 2024; 31(9):2604-2614.

PMID: 38816594 DOI: 10.1007/s43032-024-01606-8.


Reprogramming mechanism dissection and trophoblast replacement application in monkey somatic cell nuclear transfer.

Liao Z, Zhang J, Sun S, Li Y, Xu Y, Li C Nat Commun. 2024; 15(1):5.

PMID: 38228612 PMC: 10791636. DOI: 10.1038/s41467-023-43985-7.