Globular Adiponectin Augments Insulin Secretion from Pancreatic Islet Beta Cells at High Glucose Concentrations

Overview

Journal Endocrine

Specialty Endocrinology

Date 2007 Feb 27

PMID 17322583

Citations 26

Authors

Weiqiong Gu

Xiaoying Li

Changqin Liu

Jun Yang

Lei Ye

Jingfen Tang

Yuanjun Gu

Yisheng Yang

Jie Hong

Yifei Zhang

Mingdao Chen

Guang Ning

Affiliations

Soon will be listed here.

Abstract

Adiponectin plays an important role in improving insulin resistance and preventing atherosclerosis. However it has been rarely reported that adiponectin influences insulin secretion because its receptor was identified in human islet beta cells. In order to investigate the direct effect of adiponectin on pancreatic islet beta cells, we performed an insulin secretion test in purified rat islets, which were incubated with adiponectin (100 ng/mL) at low (3.3 mM) and high (16.7 mM) glucose concentrations. Furthermore, cell lysates were extracted from the adiponectin-treated islets for p-AMPKalpha assay. RTPCR and immunohistochemical examination showed both adiponectin receptor 1 (AdipoR1) and receptor 2 (AdipoR2) were expressed in islet cells and AdipoR1 was predominantly expressed. Insulin secretion was significantly increased in the presence of adiponectin for 6 h at high glucose concentration. Meanwhile, the levels of phosphorylated AMPK increased with adiponectin treatment at high glucose concentrations. It is concluded that adiponectin augments insulin secretion from pancreatic islet beta cells at high glucose concentration through AMPK activation.

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