Microbial Community Composition of the Danshui River Estuary of Northern Taiwan and the Practicality of the Phylogenetic Method in Microbial Barcoding

Overview

Journal Microb Ecol

Specialties Environmental Health
Microbiology

Date 2007 Feb 24

PMID 17318679

Citations 11

Authors

Pei-Chun Liao

Bing-Hong Huang

Shong Huang

Affiliations

Soon will be listed here.

Abstract

In this study, the microbial community in a mangrove ecosystem was surveyed and used to test the eligibility of 16S rDNA library and neighbor-joining method for the purpose of estimating microbial composition. Genetic diversity (pi) and four other diversity indices (Simpson's unbiased, Shannon-Wiener, Evenness, and Chao1 indices) were applied to estimate the adaptive lineages of microorganisms in the mangrove ecosystem. The results indicated that gamma-Proteobacteria is the most diverse taxon, while the most abundant family is Rhodobacteraceae (alpha-Proteobacteria), followed by Comamonadaceae (beta-Proteobacteria). This result may imply the existence of a graded distribution of microbial diversity across a spectrum of different salinities in the waterbody of this estuary ecosystem. Furthermore, at least 500-1,000 bps of the posterior portion of 16S rDNA is required as a marker to profile the microbial diversity in a microcosm of interest using phylogenetic methods, according to the results of our sliding window analyses for the measurements of pi, consistency index, and retention index.

Citing Articles

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