Array Painting Using Microdissected Chromosomes to Map Chromosomal Breakpoints

Overview

Journal Cytogenet Genome Res

Specialty Genetics

Date 2007 Feb 24

PMID 17317954

Citations 12

Authors

L Backx

H Van Esch

C Melotte

N Kosyakova

H STARKE

J-P Frijns

T Liehr

J R Vermeesch

Affiliations

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Abstract

Molecular characterization of breakpoints of chromosomal rearrangements is a successful strategy for the identification of candidate disease genes. Mapping translocation breakpoints and rearranged chromosomal boundaries is labor intensive and/or time consuming. Here, we present a novel and rapid procedure to map such chromosomal breakpoints by hybridizing amplified microdissection derived DNA of aberrant chromosomes to arrays containing genomic clones. We illustrate the potential of the technique by molecularly delineating the breakpoints in five small supernumerary marker chromosomes (sSMC) and mapping the breakpoints of five different chromosomal translocations.

Citing Articles

Microdissection of lampbrush chromosomes as an approach for generation of locus-specific FISH-probes and samples for high-throughput sequencing.

Zlotina A, Kulikova T, Kosyakova N, Liehr T, Krasikova A BMC Genomics. 2016; 17:126.

PMID: 26897606 PMC: 4761191. DOI: 10.1186/s12864-016-2437-4.

Clinical impact of proximal autosomal imbalances.

Hamid A, Weise A, Voigt M, Bucksch M, Kosyakova N, Liehr T Balkan J Med Genet. 2013; 15(2):15-22.

PMID: 24052727 PMC: 3776667. DOI: 10.2478/bjmg-2013-0002.

Supernumerary marker chromosomes derived from chromosome 6: cytogenetic, molecular cytogenetic, and array CGH characterization.

Huang B, Pearle P, Rauen K, Cotter P Am J Med Genet A. 2012; 158A(7):1568-73.

PMID: 22639445 PMC: 3378800. DOI: 10.1002/ajmg.a.35385.

Characterising chromosome rearrangements: recent technical advances in molecular cytogenetics.

Le Scouarnec S, Gribble S Heredity (Edinb). 2011; 108(1):75-85.

PMID: 22086080 PMC: 3238113. DOI: 10.1038/hdy.2011.100.

Chromosome 5 derived small supernumerary marker: towards a genotype/phenotype correlation of proximal chromosome 5 imbalances.

Melo J, Backx L, Vermeesch J, Santos H, Sousa A, Kosyakova N J Appl Genet. 2011; 52(2):193-200.

PMID: 21437654 DOI: 10.1007/s13353-011-0035-3.