Modulation of Bone Morphogenetic Protein (BMP) 2 Gene Expression by Sp1 Transcription Factors

Overview

Journal Gene

Specialty Molecular Biology

Date 2007 Feb 24

PMID 17317039

Citations 13

Authors

Junwang Xu

Melissa B Rogers

Affiliations

Soon will be listed here.

Abstract

Changes in Bone Morphogenetic Protein (BMP) 2 gene expression and activity have been linked to many pathological conditions including cancer, osteoarthritis, and birth defects. BMP2 gene polymorphisms have been linked to osteoporosis and osteoarthritis. Sp1 and related proteins are widely expressed regulators of gene expression whose transcription activating abilities vary in different cells and on different genes. We present data indicating that the ratio of Sp1 and Sp3 isoforms varies in cells that express or do not express BMP2. Furthermore, the orientation of Sp1 sites conserved between four orders of mammals influences BMP2 expression. Together our data indicate that the stoichiometry and orientation of Sp1 and Sp3 complexes on the BMP2 promoter influence BMP2 expression.

Citing Articles

WTAP increases BMP2 expression to promote osteoblast differentiation and inhibit osteoblast senescence via mA methylation of Sp1.

Yue B, Zhang W, Li M, Xu L Mol Genet Genomics. 2024; 299(1):109.

PMID: 39601917 DOI: 10.1007/s00438-024-02203-9.

Screening of SNP Loci Related to Leg Length Trait in Leizhou Goats Based on Whole-Genome Resequencing.

Liu J, Dong S, Lv J, Li Y, Sun B, Guo Y Int J Mol Sci. 2024; 25(22).

PMID: 39596516 PMC: 11594888. DOI: 10.3390/ijms252212450.

In Silico Analysis: Genome-Wide Identification, Characterization and Evolutionary Adaptations of Bone Morphogenetic Protein (BMP) Gene Family in Homo sapiens.

Riaz Z, Hussain M, Parveen S, Sultana M, Saeed S, Ishaque U Mol Biotechnol. 2023; 66(11):3336-3356.

PMID: 37914865 DOI: 10.1007/s12033-023-00944-3.

Effectiveness of Combination of Chitosan Gel and Hydroxyapatite from Crabs Shells () Waste as Bonegraft on Periodontal Network Regeneration through IL-1 and BMP-2 Analysis.

Gani A, Yulianty R, Supiaty S, Rusdy M, Dwipa Asri G, Eka Satya D Int J Biomater. 2022; 2022:1817236.

PMID: 35356491 PMC: 8958107. DOI: 10.1155/2022/1817236.

Identification of the specific microRNAs and competitive endogenous RNA mechanisms in osteoporosis.

Hong J, Ye F, Yu B, Gao J, Qi F, Wang W J Int Med Res. 2020; 48(10):300060520954722.

PMID: 33021861 PMC: 7543140. DOI: 10.1177/0300060520954722.

References

Valdes A, Hart D, Jones K, Surdulescu G, Swarbrick P, Doyle D . Association study of candidate genes for the prevalence and progression of knee osteoarthritis. Arthritis Rheum. 2004; 50(8):2497-507. DOI: 10.1002/art.20443. View

Urist M . Bone: formation by autoinduction. Science. 1965; 150(3698):893-9. DOI: 10.1126/science.150.3698.893. View

Gidoni D, Dynan W, Tjian R . Multiple specific contacts between a mammalian transcription factor and its cognate promoters. Nature. 1984; 312(5993):409-13. DOI: 10.1038/312409a0. View

Wozney J, Rosen V, Celeste A, Mitsock L, Whitters M, Kriz R . Novel regulators of bone formation: molecular clones and activities. Science. 1988; 242(4885):1528-34. DOI: 10.1126/science.3201241. View

Jackson S, Tjian R . Purification and analysis of RNA polymerase II transcription factors by using wheat germ agglutinin affinity chromatography. Proc Natl Acad Sci U S A. 1989; 86(6):1781-5. PMC: 286788. DOI: 10.1073/pnas.86.6.1781. View

Vasios G, Gold J, Petkovich M, Chambon P, Gudas L . A retinoic acid-responsive element is present in the 5' flanking region of the laminin B1 gene. Proc Natl Acad Sci U S A. 1989; 86(23):9099-103. PMC: 298441. DOI: 10.1073/pnas.86.23.9099. View

Lyons K, Pelton R, Hogan B . Patterns of expression of murine Vgr-1 and BMP-2a RNA suggest that transforming growth factor-beta-like genes coordinately regulate aspects of embryonic development. Genes Dev. 1989; 3(11):1657-68. DOI: 10.1101/gad.3.11.1657. View

Lyons K, Pelton R, Hogan B . Organogenesis and pattern formation in the mouse: RNA distribution patterns suggest a role for bone morphogenetic protein-2A (BMP-2A). Development. 1990; 109(4):833-44. DOI: 10.1242/dev.109.4.833. View

Devlin R, Du Z, Pereira R, Kimble R, Economides A, Jorgetti V . Skeletal overexpression of noggin results in osteopenia and reduced bone formation. Endocrinology. 2003; 144(5):1972-8. DOI: 10.1210/en.2002-220918. View

10.

Canalis E, Economides A, Gazzerro E . Bone morphogenetic proteins, their antagonists, and the skeleton. Endocr Rev. 2003; 24(2):218-35. DOI: 10.1210/er.2002-0023. View

11.

Hill C . Turning off Smads: identification of a Smad phosphatase. Dev Cell. 2006; 10(4):412-3. DOI: 10.1016/j.devcel.2006.03.008. View

12.

Yatani A, Shen Y, Yan L, Chen W, Kim S, Sano K . Down regulation of the L-type Ca2+ channel, GRK2, and phosphorylated phospholamban: protective mechanisms for the denervated failing heart. J Mol Cell Cardiol. 2006; 40(5):619-28. DOI: 10.1016/j.yjmcc.2006.02.002. View

13.

Fritz D, Jiang S, Xu J, Rogers M . A polymorphism in a conserved posttranscriptional regulatory motif alters bone morphogenetic protein 2 (BMP2) RNA:protein interactions. Mol Endocrinol. 2006; 20(7):1574-86. DOI: 10.1210/me.2005-0469. View

14.

Schlueter J, Manner J, Brand T . BMP is an important regulator of proepicardial identity in the chick embryo. Dev Biol. 2006; 295(2):546-58. DOI: 10.1016/j.ydbio.2006.03.036. View

15.

Xiong D, Shen H, Zhao L, Xiao P, Yang T, Guo Y . Robust and comprehensive analysis of 20 osteoporosis candidate genes by very high-density single-nucleotide polymorphism screen among 405 white nuclear families identified significant association and gene-gene interaction. J Bone Miner Res. 2006; 21(11):1678-95. PMC: 1829486. DOI: 10.1359/jbmr.060808. View

16.

Tsuji K, Bandyopadhyay A, Harfe B, Cox K, Kakar S, Gerstenfeld L . BMP2 activity, although dispensable for bone formation, is required for the initiation of fracture healing. Nat Genet. 2006; 38(12):1424-9. DOI: 10.1038/ng1916. View

17.

Rodriguez-Leon J, Merino R, Macias D, Ganan Y, Santesteban E, Hurle J . Retinoic acid regulates programmed cell death through BMP signalling. Nat Cell Biol. 1999; 1(2):125-6. DOI: 10.1038/10098. View

18.

Celeste A, Iannazzi J, Taylor R, Hewick R, Rosen V, Wang E . Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone. Proc Natl Acad Sci U S A. 1990; 87(24):9843-7. PMC: 55270. DOI: 10.1073/pnas.87.24.9843. View

19.

Rogers M, Rosen V, Wozney J, Gudas L . Bone morphogenetic proteins-2 and -4 are involved in the retinoic acid-induced differentiation of embryonal carcinoma cells. Mol Biol Cell. 1992; 3(2):189-96. PMC: 275518. DOI: 10.1091/mbc.3.2.189. View

20.

Masson N, Hurst H, Lee K . Identification of proteins that interact with CREB during differentiation of F9 embryonal carcinoma cells. Nucleic Acids Res. 1993; 21(11):1163-9. PMC: 309277. DOI: 10.1093/nar/21.5.1163. View