Hepatitis B Virus (HBV)-specific Cytotoxic T-cell (CTL) Response in Humans: Characterization of HLA Class II-restricted CTLs That Recognize Endogenously Synthesized HBV Envelope Antigens

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1992 Feb 1

PMID 1731098

Citations 14

Authors

A Penna

P Fowler

A Bertoletti

S Guilhot

B Moss

R F Margolskee

A Cavalli

A Valli

F Fiaccadori

F V Chisari

Affiliations

Soon will be listed here.

Abstract

In this study, we show that CD4+, hepatitis B virus (HBV) envelope-specific T-cell clones produced by stimulation with a particulate antigen preparation are able to recognize and kill not only autologous antigen-presenting cells incubated with exogenous HBV envelope antigens but also autologous HLA class II-positive cells expressing endogenously synthesized HBV envelope antigens following infection with recombinant vaccinia viruses or transfection with recombinant Epstein-Barr virus expression vectors. Experiments with lysosomotropic agents and brefeldin A suggest that the endosomal compartment is likely involved in the processing of endogenously synthesized viral proteins for recognition by CD4+ T cells. Our study indicates that HBV envelope-specific, HLA class II-restricted CD4+ cytotoxic T lymphocytes can potentially participate in the immune clearance of HBV-infected cells and the pathogenesis of hepatocellular injury in hepatitis B.

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