» Articles » PMID: 17306787

Elevated Apoptosis-associated Cytokeratin 18 Fragments (CK18Asp386) in Serum of Patients with Chronic Liver Diseases Indicate Hepatic and Biliary Inflammation

Overview
Journal Clin Biochem
Specialty Biochemistry
Date 2007 Feb 20
PMID 17306787
Citations 21
Authors
Affiliations
Soon will be listed here.
Abstract

Objectives: During apoptosis, intermediate filament protein cytokeratin 18 (CK18) is cleaved by caspases at Asp396 which can be specifically detected by the monoclonal antibody M30 (M30-antigen).

Design And Methods: M30-antigen serum levels were analyzed in 76 chronic liver diseases (CLD) patients and 62 healthy controls.

Results: M30-antigen levels were significantly elevated in CLD patients (median 296.3 U/L) compared with healthy controls (median 153.5 U/L, P<0.001) and increased with disease severity (Child-Pugh or MELD score). M30-antigen correlated with aminotransferase activities and parameters indicating cholestasis such as bile acids. Highest serum M30-antigen was associated with histologically confirmed severe intrahepatic cholestasis (median 599.1 U/L) or biliary duct inflammation (median 648.0 U/L). Furthermore, in contrast to patients with liver cirrhosis, presence of hepatocellular carcinoma was associated with elevated M30-antigen in patients without cirrhosis.

Conclusion: Serum M30-antigen levels are elevated in CLD and correlate with hepatic inflammation as well as cholangitis and cholestasis.

Citing Articles

MAFLD/NAFLD Biopsy-Free Scoring Systems for Hepatic Steatosis, NASH, and Fibrosis Diagnosis.

Segura-Azuara N, Varela-Chinchilla C, Trinidad-Calderon P Front Med (Lausanne). 2022; 8:774079.

PMID: 35096868 PMC: 8792949. DOI: 10.3389/fmed.2021.774079.


The application of cytokeratin-18 as a biomarker for drug-induced liver injury.

Korver S, Bowen J, Pearson K, Gonzalez R, French N, Park K Arch Toxicol. 2021; 95(11):3435-3448.

PMID: 34322741 PMC: 8492595. DOI: 10.1007/s00204-021-03121-0.


Time-Course Changes of Serum Keratin Concentrations after Liver Transplantation: Contrasting Results of Keratin-18 and Keratin-19 Fragments.

Macia C, Loureiro J, Campos-Varela I, Abdulkader I, Otero E, Varo E Case Reports Hepatol. 2020; 2020:8895435.

PMID: 33335785 PMC: 7723486. DOI: 10.1155/2020/8895435.


Individualized risk prediction of significant fibrosis in non-alcoholic fatty liver disease using a novel nomogram.

Zhou Y, Ye F, Li Y, Pan X, Chen Y, Wu X United European Gastroenterol J. 2019; 7(8):1124-1134.

PMID: 31662869 PMC: 6794687. DOI: 10.1177/2050640619868352.


The efficacy of serum cell death biomarkers for diagnosing biliary tract cancer.

Sugimoto M, Abe K, Hayashi M, Takagi T, Suzuki R, Konno N Sci Rep. 2018; 8(1):16997.

PMID: 30451962 PMC: 6243019. DOI: 10.1038/s41598-018-35278-7.