The Catalytic Subunit of DNA-protein Kinase (DNA-PKcs) is Not Required for Ig Class-switch Recombination

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2007 Feb 14

PMID 17296939

Citations 13

Authors

Kerstin Kiefer

Jennifer Oshinsky

Jiyoon Kim

Pamela B Nakajima

Gayle C Bosma

Melvin J Bosma

Affiliations

Soon will be listed here.

Abstract

The joining of DNA ends during Ig class-switch recombination (CSR) is thought to involve the same nonhomologous end-joining pathway as used in V(D)J recombination. However, we reported earlier that CSR can readily occur in Ig transgenic SCID mice lacking DNA-dependent protein kinase (DNA-PK) activity, a critical enzymatic activity for V(D)J recombination. We were thus led to question whether the catalytic subunit of DNA-PK (DNA-PKcs) is essential for CSR. To address this issue, we asked whether class switching to different Ig isotypes could occur in a line of Ig transgenic mice lacking detectable DNA-PKcs protein. The answer was affirmative. We conclude that joining of DNA ends during CSR does not require DNA-PKcs and can occur by an alternative repair pathway to that used for V(D)J recombination.

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