Improved Expression of Recombinant GFP Using a Replicating Vector Based on Beet Curly Top Virus in Leaf-disks and Infiltrated Nicotiana Benthamiana Leaves

Overview

Journal Plant Mol Biol

Date 2007 Feb 13

PMID 17294255

Citations 14

Authors

Kyung Il Kim

Garry Sunter

David M Bisaro

In Sik Chung

Affiliations

Soon will be listed here.

Abstract

Recombinant green fluorescent protein (GFP) with a molecular mass of 29 kDa was transiently expressed in Agrobacterium-inoculated leaf-disks prepared from Nicotiana benthamiana plants. Expression of GFP from the Cauliflower mosaic virus (CaMV) 35 S promoter within a replicating vector based on the geminivirus Beet curly top virus (BCTV) was more than 3 times higher than from a control, non-replicating vector. Use of the Cassava vein mosaic virus (CsVMV) promoter in the BCTV replicating vector increased the expression of recombinant GFP 320% at the transcript level, compared to use of the control CaMV 35 S promoter. Expression of recombinant GFP from Agrobacterium-inoculated leaf-disks of N. benthamiana was further enhanced up to 240% in the presence of post-transcriptional gene silencing suppressor p19.

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