Regulation of the Salmonella Typhimurium MetA Gene by the MetR Protein and Homocysteine

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1992 Jan 1

PMID 1729233

Citations 22

Authors

R Mares

M L Urbanowski

G V Stauffer

Affiliations

Soon will be listed here.

Abstract

The DNA sequence of the Salmonella typhimurium metA control region is presented. S1 nuclease mapping was used to determine the transcription initiation site. By measuring beta-galactosidase levels in Escherichia coli strains lysogenized with lambda phage carrying a metA-lacZ gene fusion, the MetR protein was shown to activate the metA gene. Homocysteine, an intermediate in methionine biosynthesis, plays a negative role in the MetR-mediated activation mechanism. Gel mobility shift assays and DNase I protection experiments showed that the MetR protein binds to a DNA fragment carrying the metA control region and protects a 26-bp region beginning 9 bp upstream of the -35 promoter sequence.

Citing Articles

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Pan X, Sun C, Tang M, You J, Osire T, Zhao Y Appl Environ Microbiol. 2019; 86(4).

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Methionine-mediated gene expression and characterization of the CmhR regulon in .

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Cysteine-Mediated Gene Expression and Characterization of the CmbR Regulon in .

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Structural basis for DNA recognition by the transcription regulator MetR.

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Comparative genomics of transcriptional regulation of methionine metabolism in Proteobacteria.

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