Increased Polyploidy in Aortic Vascular Smooth Muscle Cells During Aging is Marked by Cellular Senescence

Overview

Journal Aging Cell

Specialties Cell Biology
Geriatrics

Date 2007 Feb 13

PMID 17291294

Citations 29

Authors

Dan Yang

Donald J McCrann

Hao Nguyen

Cynthia St Hilaire

Ronald A DePinho

Matthew R Jones

Katya Ravid

Affiliations

Soon will be listed here.

Abstract

We previously reported that the frequency of polyploid aortic vascular smooth muscle cells (VSMC) serves as a biomarker of aging. Cellular senescence of somatic cells is another marker of aging that is characterized by the inability to undergo cell division. Here, we examined whether polyploidy is associated with the development of cellular senescence in vivo. Analysis of aortic tissue preparations from young and old Brown Norway rats showed that expression of senescence markers such as p16(INK4a) and senescence-associated beta-galactosidase activity are detected primarily in the old tissues. VSMC from p16(INK4a) knockout and control mice display similar levels of polyploid cells. Intriguingly, senescence markers are expressed in most, but not all, polyploid VSMC. Moreover, the polyploid cells exhibit limited proliferative capacity in comparison to their diploid counterparts. This study is the first to demonstrate in vivo that polyploid VSMC adopt a senescent phenotype.

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