Effective Inhibition of Porcine Transmissible Gastroenteritis Virus Replication in ST Cells by ShRNAs Targeting RNA-dependent RNA Polymerase Gene

Overview

Journal Antiviral Res

Publisher Elsevier

Date 2007 Feb 9

PMID 17287033

Citations 10

Authors

Jun-Fang Zhou

Xiu-guo Hua

Li Cui

Jian-Guo Zhu

De-nian Miao

Yong Zou

Xi-zhong He

Wan-guo Su

Affiliations

Soon will be listed here.

Abstract

Transmissible gastroenteritis virus (TGEV) is identified as one of the most important pathogenic agents during swine enteric infection, leading to high mortality in neonatal pigs and severe annual economic loss in swine-producing areas. Up to date, various vaccines developed against TGEV still need to be improved. To exploit the possibility of using RNA interference (RNAi) as a strategy against TGEV infection, two shRNA-expressing plasmids (pEGFP-U6/P1 and pEGFP-U6/P2) targeting the RNA-dependent RNA polymerase (RdRp) gene of TGEV were constructed and transfected into swine testicular (ST) cells. The cytopathic effect (CPE) and MTS assays demonstrated that both shRNAs were capable of protecting cells against TGEV invasion with very high specificity and efficiency. A real-time quantitative RT-PCR further confirmed that the amounts of viral RNAs in cell cultures pre-transfected with the two plasmids were reduced by 95.2% and up to 100%, respectively. Our results suggest that RNAi might be a promising new strategy against TGEV infection.

Citing Articles

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