Discrimination of Cryptosporidium Species by Denaturing Gradient Gel Electrophoresis

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2007 Feb 7

PMID 17279394

Authors

Masaaki Satoh

Yutaka Nakai

Affiliations

Soon will be listed here.

Abstract

Denaturing gradient gel electrophoresis (DGGE) was used for the discrimination of three species and one genotype of the protozoan parasite Cryptosporidium: the C. parvum, C. andersoni, C. muris, and C. muris Japanese field mouse genotype. A set of primers specific for the 18S rRNA gene of Cryptosporidium was used in the DGGE; consequently, the four strains showed different banding patterns. This is a potentially convenient and precise method for the discrimination of Cryptosporidium spp.

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