MEPE Expression in Osteocytes During Orthodontic Tooth Movement

Overview

Journal Arch Oral Biol

Specialty Dentistry

Date 2007 Feb 3

PMID 17270144

Citations 31

Authors

J Gluhak-Heinrich

D Pavlin

W Yang

M MacDougall

S E Harris

Affiliations

Soon will be listed here.

Abstract

Unlabelled: MEPE and DMP1 may play a role in mineralisation and demineralisation within the osteocyte microenvironment. Our earlier studies showed that DMP1 is mechanically responsive [Gluhak-Heinrich J, Ye L, Bonewald LF, Feng JQ, MacDougall M, Harris SE, et al. Mechanical loading stimulates dentin matrix protein 1 (DMP1) in osteocytes in vivo. J Bone Min Res 2003;18(5):807-17].

Objectives: To examine the effect of mechanical loading on the expression of MEPE using mouse tooth movement model, and compare this effect to that on DMP1.

Methods: In situ hybridisation and immunohistochemistry was performed on 38 treated and 38 control bone sites loaded 6-72 h. ImageJ was used for quantification of mRNA expression in osteocytes.

Results: Alveolar osteocytes showed high basal level of MEPE that decreased during the first day of loading, followed by 2.8-fold stimulation at day 3, and returning to a control level by day 7.

Conclusion: The osteocyte specific mechanical stimulation of MEPE was delayed and different, compared to that of DMP1. This suggests a distinct role of MEPE and DMP1 in the response of osteocytes to mechanical loading in vivo.

Citing Articles

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Mechanical loading modulates phosphate related genes in rat bone.

Nepal A, van Essen H, Reijnders C, Lips P, Bravenboer N PLoS One. 2023; 18(3):e0282678.

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Calcium‑dependent activation of PHEX, MEPE and DMP1 in osteocytes.

Donmez B, Karagur E, Cort Donmez A, Choi J, Akkus O Mol Med Rep. 2022; 26(6).

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Choi J, Kijas A, Lauko J, Rowan A Front Cell Dev Biol. 2022; 9:770143.

PMID: 35265628 PMC: 8900535. DOI: 10.3389/fcell.2021.770143.

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