Salsolinol, an Endogenous Neurotoxin, Activates JNK and NF-kappaB Signaling Pathways in Human Neuroblastoma Cells

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 2007 Feb 3

PMID 17268850

Citations 5

Authors

Sawitri Wanpen

Patcharee Kooncumchoo

Shaik Shavali

Piyarat Govitrapong

Manuchair Ebadi

Affiliations

Soon will be listed here.

Abstract

Salsolinol, an endogenous neurotoxin, is known to be involved in the pathogenesis of Parkinson's disease (PD). In the present study, we have investigated the effects of salsolinol on the activation of two different signaling pathways that involve c-Jun N-terminal kinase (JNK), and nuclear factor-kappaB, (NF-kappaB) in human dopaminergic neuroblastoma SH-SY5Y cells. Salsolinol treatment caused upregulation in the levels of c-Jun and phosphorylated c-Jun. It also caused degradation of IkappaBalpha and translocated the active NF-kappaB into the nucleus. The binding activity of NF-kappaB to DNA was enhanced by salsolinol in a concentration dependent manner. Furthermore, salsolinol decreased the levels of the anti-apoptotic protein Bcl-2, and increased pro-apoptotic protein Bax, while enhancing the release of cytochrome-c from mitochondria. Mitochondrial complex-I activity was significantly decreased and reactive oxygen species (ROS) were increased in salsolinol treated cells. These results partly suggest that salsolinol-induced JNK and NF-kappaB signaling pathways may be involved in induction of apoptosis in human dopaminergic neurons, as seen in Parkinson's disease.

Citing Articles

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