Cryopreservation of Hematopoietic Stem Cells

Overview

Journal Am J Hematol

Specialty Hematology

Date 2007 Feb 3

PMID 17266054

Citations 70

Authors

David Berz

Elise M McCormack

Eric S Winer

Gerald A Colvin

Peter J Quesenberry

Affiliations

Soon will be listed here.

Abstract

Stem cell transplantation represents a critical approach for the treatment of many malignant and non-malignant diseases. The foundation for these approaches is the ability to cryopreserve marrow cells for future use. This technique is routinely employed in all autologous settings and is critical for cord blood transplantation. A variety of cryopreservatives have been used with multiple freezing and thawing techniques as outlined in the later chapters. Freezing efficiency has been proven repeatedly and the ability of long-term stored marrow to repopulate has been established. Standard approaches outlined here are used in many labs as the field continues to evolve.

Citing Articles

Orthobiologic Products: Preservation Options for Orthopedic Research and Clinical Applications.

Fang W, Vangsness Jr C J Clin Med. 2024; 13(21).

PMID: 39518716 PMC: 11546119. DOI: 10.3390/jcm13216577.

Trehalose in cryopreservation. Applications, mechanisms and intracellular delivery opportunities.

Murray A, Kilbride P, Gibson M RSC Med Chem. 2024; 15(9):2980-2995.

PMID: 39309363 PMC: 11411628. DOI: 10.1039/d4md00174e.

Freezing biological organisms for biomedical applications.

Kuang G, Zhang Q, Jia J, Yu Y Smart Med. 2024; 1(1):e20220034.

PMID: 39188743 PMC: 11235656. DOI: 10.1002/SMMD.20220034.

Engineering a Microfluidic Platform to Cryopreserve Stem Cells: A DMSO-Free Sustainable Approach.

Modaresi S, Pacelli S, Chakraborty A, Coyle A, Luo W, Singh I Adv Healthc Mater. 2024; 13(29):e2401264.

PMID: 39152923 PMC: 11582517. DOI: 10.1002/adhm.202401264.

Key quality parameter comparison of mesenchymal stem cell product cryopreserved in different cryopreservation solutions for clinical applications.

Tan Y, Salkhordeh M, Murray A, Souza-Moreira L, Stewart D, Mei S Front Bioeng Biotechnol. 2024; 12:1412811.

PMID: 39148941 PMC: 11324487. DOI: 10.3389/fbioe.2024.1412811.

References

Walter Z, Szostek M, Weglarska D, Raguszewska D, Jablonski M, Lorenz F . [Methods for freezing, thawing and viability estimation of hemopoietic stem cells]. Przegl Lek. 1999; 56 Suppl 1:34-9. View

Prince H, Page S, Keating A, Saragosa R, Vukovic N, Imrie K . Microbial contamination of harvested bone marrow and peripheral blood. Bone Marrow Transplant. 1995; 15(1):87-91. View

Villalon L, Odriozola J, Ramos P, Ramos M, Herrera P, Perez de Oteyza J . Cryopreserving with increased cellular concentrations of peripheral blood progenitor cells: clinical results. Haematologica. 2002; 87(2):ELT06. View

Davis J, Rowley S, Braine H, Piantadosi S, SANTOS G . Clinical toxicity of cryopreserved bone marrow graft infusion. Blood. 1990; 75(3):781-6. View

Ketheesan N, Whiteman C, Malczewski A, Hirst R, La Brooy J . Effect of cryopreservation on the immunogenicity of umbilical cord blood cells. Transfus Apher Sci. 2004; 30(1):47-54. DOI: 10.1016/j.transci.2003.05.002. View

Stroh C, Cassens U, Samraj A, Sibrowski W, Schulze-Osthoff K, Los M . The role of caspases in cryoinjury: caspase inhibition strongly improves the recovery of cryopreserved hematopoietic and other cells. FASEB J. 2002; 16(12):1651-3. DOI: 10.1096/fj.02-0034fje. View

Schafer T, Everett J, Silver G, Came P . Biohazard: virus-contaminated liquid nitrogen. Science. 1976; 191(4222):24-6. DOI: 10.1126/science.191.4222.24-c. View

Bakken A, Bruserud O, Abrahamsen J . No differences in colony formation of peripheral blood stem cells frozen with 5% or 10% dimethyl sulfoxide. J Hematother Stem Cell Res. 2003; 12(3):351-8. DOI: 10.1089/152581603322023089. View

MERYMAN H, Williams R, Douglas M . Freezing injury from "solution effects" and its prevention by natural or artificial cryoprotection. Cryobiology. 1977; 14(3):287-302. DOI: 10.1016/0011-2240(77)90177-8. View

10.

Milosevic J, Storch A, Schwarz J . Cryopreservation does not affect proliferation and multipotency of murine neural precursor cells. Stem Cells. 2005; 23(5):681-8. DOI: 10.1634/stemcells.2004-0135. View

11.

Nagamura-Inoue T, Shioya M, Sugo M, Cui Y, Takahashi A, Tomita S . Wash-out of DMSO does not improve the speed of engraftment of cord blood transplantation: follow-up of 46 adult patients with units shipped from a single cord blood bank. Transfusion. 2003; 43(9):1285-95. DOI: 10.1046/j.1537-2995.2003.00486.x. View

12.

Broxmeyer H, Srour E, Hangoc G, Cooper S, Anderson S, Bodine D . High-efficiency recovery of functional hematopoietic progenitor and stem cells from human cord blood cryopreserved for 15 years. Proc Natl Acad Sci U S A. 2003; 100(2):645-50. PMC: 141050. DOI: 10.1073/pnas.0237086100. View

13.

Matsumoto N, Yoshizawa H, Kagamu H, Abe T, Fujita N, Watanabe S . Successful liquid storage of peripheral blood stem cells at subzero non-freezing temperature. Bone Marrow Transplant. 2002; 30(11):777-84. DOI: 10.1038/sj.bmt.1703692. View

14.

Cabezudo E, Dalmases C, Ruz M, Sanchez J, Torrico C, Sola C . Leukapheresis components may be cryopreserved at high cell concentrations without additional loss of HPC function. Transfusion. 2000; 40(10):1223-7. DOI: 10.1046/j.1537-2995.2000.40101223.x. View

15.

Rodriguez L, Velasco B, Garcia J, Martin-Henao G . Evaluation of an automated cell processing device to reduce the dimethyl sulfoxide from hematopoietic grafts after thawing. Transfusion. 2005; 45(8):1391-7. DOI: 10.1111/j.1537-2995.2005.00213.x. View

16.

Halle P, Tournilhac O, Knopinska-Posluszny W, Kanold J, Gembara P, Boiret N . Uncontrolled-rate freezing and storage at -80 degrees C, with only 3.5-percent DMSO in cryoprotective solution for 109 autologous peripheral blood progenitor cell transplantations. Transfusion. 2001; 41(5):667-73. DOI: 10.1046/j.1537-2995.2001.41050667.x. View

17.

Bock T, Orlic D, Dunbar C, Broxmeyer H, Bodine D . Improved engraftment of human hematopoietic cells in severe combined immunodeficient (SCID) mice carrying human cytokine transgenes. J Exp Med. 1995; 182(6):2037-43. PMC: 2192238. DOI: 10.1084/jem.182.6.2037. View

18.

Zambelli A, Poggi G, Da Prada G, Pedrazzoli P, Cuomo A, Miotti D . Clinical toxicity of cryopreserved circulating progenitor cells infusion. Anticancer Res. 1999; 18(6B):4705-8. View

19.

Buchanan S, Gross S, Acker J, Toner M, Carpenter J, Pyatt D . Cryopreservation of stem cells using trehalose: evaluation of the method using a human hematopoietic cell line. Stem Cells Dev. 2004; 13(3):295-305. DOI: 10.1089/154732804323099226. View

20.

Kurtzberg J, Laughlin M, Graham M, Smith C, Olson J, Halperin E . Placental blood as a source of hematopoietic stem cells for transplantation into unrelated recipients. N Engl J Med. 1996; 335(3):157-66. DOI: 10.1056/NEJM199607183350303. View