Isolation and Characterization of Endophytic Bacteria from the Nickel Hyperaccumulator Plant Alyssum Bertolonii

Overview

Journal Microb Ecol

Specialties Environmental Health
Microbiology

Date 2007 Feb 1

PMID 17264998

Citations 33

Authors

Rita Barzanti

Francesca Ozino

Marco Bazzicalupo

Roberto Gabbrielli

Francesca Galardi

Cristina Gonnelli

Alessio Mengoni

Affiliations

Soon will be listed here.

Abstract

We report the isolation and characterization of endophytic bacteria, endemic to serpentine outcrops of Central Italy, from a nickel hyperaccumulator plant, Alyssum bertolonii Desv. (Brassicaceae). Eighty-three endophytic bacteria were isolated from roots, stems, and leaves of A. bertolonii and classified by restriction analysis of 16S rDNA (ARDRA) and partial 16S rDNA sequencing in 23 different taxonomic groups. All isolates were then screened for siderophore production and for resistance to heavy metals. One isolate representative of each ARDRA group was then tested for plant tissue colonization ability in sterile culture. Obtained results pointed out that, despite the high concentration of heavy metals present in its tissues, A. bertolonii harbors an endophytic bacterial flora showing a high genetic diversity as well as a high level of resistance to heavy metals that could potentially help plant growth and Ni hyperaccumulation.

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