Type I Gamma Phosphatidylinositol Phosphate Kinase Modulates Adherens Junction and E-cadherin Trafficking Via a Direct Interaction with Mu 1B Adaptin

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2007 Jan 31

PMID 17261850

Citations 103

Authors

Kun Ling

Shawn F Bairstow

Chateen Carbonara

Dmitry A Turbin

David G Huntsman

Richard A Anderson

Affiliations

Soon will be listed here.

Abstract

Assembly of E-cadherin-based adherens junctions (AJ) is obligatory for establishment of polarized epithelia and plays a key role in repressing the invasiveness of many carcinomas. Here we show that type Igamma phosphatidylinositol phosphate kinase (PIPKIgamma) directly binds to E-cadherin and modulates E-cadherin trafficking. PIPKIgamma also interacts with the mu subunits of clathrin adaptor protein (AP) complexes and acts as a signalling scaffold that links AP complexes to E-cadherin. Depletion of PIPKIgamma or disruption of PIPKIgamma binding to either E-cadherin or AP complexes results in defects in E-cadherin transport and blocks AJ assembly. An E-cadherin germline mutation that loses PIPKIgamma binding and shows disrupted basolateral membrane targeting no longer forms AJs and leads to hereditary gastric cancers. These combined results reveal a novel mechanism where PIPKIgamma serves as both a scaffold, which links E-cadherin to AP complexes and the trafficking machinery, and a regulator of trafficking events via the spatial generation of phosphatidylinositol-4,5-bisphosphate.

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