» Articles » PMID: 17246338

Genetic Fine Structure of the BRONZE Locus in Maize

Overview
Journal Genetics
Specialty Genetics
Date 1986 Aug 1
PMID 17246338
Citations 71
Authors
Affiliations
Soon will be listed here.
Abstract

The bronze (bz) locus in maize, located in the short arm of chromosome 9 (9S), is the structural gene for the anthocyanin biosynthetic enzyme UFGT. The gene has been cloned and its physical map has been oriented relative to the centromere of 9S. We report here the genetic fine structure mapping of several biochemically characterized EMS-induced bz-E mutations, derived from the Bz-W22 isoallele, and Ds insertion bz-m mutations, derived from the Bz-McC isoallele. Two UFGT(-), CRM(+ ) mutants (bz-E2 and bz-E5), which genetically identify coding sequences in the gene, and three UFGT(-), CRM(- )bz-E mutants were mapped against the Ds insertion mutants bz-m1 and bz-m2(DI) by selecting Bz intragenic recombinants from heterozygotes of the type bz-E/bz-m . The exclusive occurrence of one recombinant outside marker class allowed the unambiguous placement of the mutants in a genetic fine structure map. Peculiarly, the two CRM(+)bz-E mutants lie upstream of the three CRM(-)bz-E mutants and at a considerable genetic distance. The UFGT allozymes encoded by the progenitor alleles Bz-W22 and Bz-McC differ in two properties, thermal stability and activity. The sites responsible for these properties were mapped as unselected markers among the Bz intragenic recombinants. The thermal stability site, which also identifies a coding region of the gene, mapped very close to the CRM(+)bz-E mutant sites. The site responsible for variation in activity, which probably identifies a region involved in regulation of expression of the bz locus, mapped at the 5' or proximal end of the locus. It was found to be inseparable from the Ds insertion in bz-m1 that lies very close to the 5' end of the transcribed region.-Evidence was obtained that the insertion of Ds within the bz gene has a suppressing effect on intragenic recombination. Additional data are also presented supporting our observation that Ds affects the pattern of intragenic recombination at bz.-Based on the total genetic length of the bz gene and on the physical size of the transcribed region, we estimate that one unit of recombination at bronze corresponds to 14 kb of DNA. This estimate is more than 100 times smaller than the average value for the whole genome and implies that there may be regions, such as bronze, that serve as hotspots for recombination.

Citing Articles

Genetic diversity and population structure of modern wheat (Triticum aestivum L.) cultivars in Henan Province of China based on SNP markers.

Tang W, Dong Z, Gao L, Wang X, Li T, Sun C BMC Plant Biol. 2023; 23(1):542.

PMID: 37924000 PMC: 10625233. DOI: 10.1186/s12870-023-04537-9.


MSH2 stimulates interfering and inhibits non-interfering crossovers in response to genetic polymorphism.

Dluzewska J, Dziegielewski W, Szymanska-Lejman M, Gazecka M, Henderson I, Higgins J Nat Commun. 2023; 14(1):6716.

PMID: 37872134 PMC: 10593791. DOI: 10.1038/s41467-023-42511-z.


The chromatin determinants and Ph1 gene effect at wheat sites with contrasting recombination frequency.

Majka M, Janakova E, Jakobson I, Jarve K, Capal P, Korchanova Z J Adv Res. 2023; 53:75-85.

PMID: 36632886 PMC: 10658417. DOI: 10.1016/j.jare.2023.01.002.


The effect of DNA polymorphisms and natural variation on crossover hotspot activity in Arabidopsis hybrids.

Szymanska-Lejman M, Dziegielewski W, Dluzewska J, Kbiri N, Bieluszewska A, Poethig R Nat Commun. 2023; 14(1):33.

PMID: 36596804 PMC: 9810609. DOI: 10.1038/s41467-022-35722-3.


MSH2 shapes the meiotic crossover landscape in relation to interhomolog polymorphism in Arabidopsis.

Blackwell A, Dluzewska J, Szymanska-Lejman M, Desjardins S, Tock A, Kbiri N EMBO J. 2020; 39(21):e104858.

PMID: 32935357 PMC: 7604573. DOI: 10.15252/embj.2020104858.


References
1.
Dooner H, Nelson O . Controlling element-induced alterations in UDPglucose:flavonoid glucosyltransferase, the enzyme specified by the bronze locus in maize. Proc Natl Acad Sci U S A. 1977; 74(12):5623-7. PMC: 431836. DOI: 10.1073/pnas.74.12.5623. View

2.
Fedoroff N, Furtek D, Nelson O . Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac). Proc Natl Acad Sci U S A. 1984; 81(12):3825-9. PMC: 345313. DOI: 10.1073/pnas.81.12.3825. View

3.
Dooner H, Nelson O . Interaction among C, R and Vp in the Control of the Bz Glucosyltransferase during Endosperm Development in Maize. Genetics. 1979; 91(2):309-15. PMC: 1216368. DOI: 10.1093/genetics/91.2.309. View

4.
Dooner H, Kermicle J . The Transposable Element Ds Affects the Pattern of Intragenic Recombination at the bz and R Loci in Maize. Genetics. 1986; 113(1):135-43. PMC: 1202793. DOI: 10.1093/genetics/113.1.135. View

5.
Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, DOONER H . mRNA transcripts of several plant genes are polyadenylated at multiple sites in vivo. Nucleic Acids Res. 1986; 14(5):2229-40. PMC: 339654. DOI: 10.1093/nar/14.5.2229. View