Identification and Characterisation of Tomato Torrado Virus, a New Plant Picorna-like Virus from Tomato

Overview

Journal Arch Virol

Specialty Microbiology

Date 2007 Jan 18

PMID 17226066

Citations 18

Authors

M Verbeek

A M Dullemans

J F J M van den Heuvel

P C Maris

R A A van der Vlugt

Affiliations

Soon will be listed here.

Abstract

A new virus was isolated from tomato plants from the Murcia region in Spain which showed symptoms of 'torrado disease'; very distinct necrotic, almost burn-like symptoms on leaves of infected plants. The virus particles are isometric with a diameter of approximately 28 nm. The viral genome consists of two (+)ssRNA molecules of 7793 (RNA1) and 5389 nts (RNA2). RNA1 contains one open reading frame (ORF) encoding a predicted polyprotein of 241 kDa that shows conserved regions with motifs typical for a protease-cofactor, a helicase, a protease and an RNA-dependent RNA polymerase. RNA2 contains two, partially overlapping ORFs potentially encoding proteins of 20 and 134 kDa. These viral RNAs are encapsidated by three proteins with estimated sizes of 35, 26 and 23 kDa. Direct protein sequencing mapped these coat proteins to ORF2 on RNA2. Phylogenetic analyses of nucleotide and derived amino acid sequences showed that the virus is related to but distinct from viruses belonging to the genera Sequivirus, Sadwavirus and Cheravirus. This new virus, for which the name tomato torrado virus is proposed, most likely represents a member of a new plant virus genus.

Citing Articles

Genomic Analysis of a Novel "Rehmannia Torradovirus Virus": Two Distinct Variants Infecting .

Qin Y, Lu S, Wen Y, Li S, Gao S, Zhang D Microorganisms. 2024; 12(8).

PMID: 39203485 PMC: 11356386. DOI: 10.3390/microorganisms12081643.

Genomic characterization of a novel torradovirus infecting Arctium lappa L. in China.

Li Z, Zhang L, Fan X, Liang Q, Chen Y, Li M Arch Virol. 2023; 168(4):107.

PMID: 36899282 DOI: 10.1007/s00705-023-05736-3.

Enhanced Apiaceous Potyvirus Phylogeny, Novel Viruses, and New Country and Host Records from Sequencing Samples.

Fox A, Gibbs A, Fowkes A, Pufal H, McGreig S, Jones R Plants (Basel). 2022; 11(15).

PMID: 35956429 PMC: 9370115. DOI: 10.3390/plants11151951.

A Novel Distinct Genetic Variant of Tomato Torrado Virus with Substantially Shorter RNA1-Specific 3'Untranslated Region (3'UTR).

Budziszewska M, Wieczorek P Plants (Basel). 2021; 10(11).

PMID: 34834816 PMC: 8621019. DOI: 10.3390/plants10112454.

Global Advances in Tomato Virome Research: Current Status and the Impact of High-Throughput Sequencing.

Rivarez M, Vucurovic A, Mehle N, Ravnikar M, Kutnjak D Front Microbiol. 2021; 12:671925.

PMID: 34093492 PMC: 8175903. DOI: 10.3389/fmicb.2021.671925.

References

Li C, Yoshikawa N, Takahashi T, Ito T, Yoshida K, Koganezawa H . Nucleotide sequence and genome organization of apple latent spherical virus: a new virus classified into the family Comoviridae. J Gen Virol. 2000; 81(Pt 2):541-7. DOI: 10.1099/0022-1317-81-2-541. View

Candresse T, Svanella-Dumas L, Gall O . Characterization and partial genome sequence of stocky prune virus, a new member of the genus Cheravirus. Arch Virol. 2005; 151(6):1179-88. DOI: 10.1007/s00705-005-0682-y. View

Volpicella M, Ceci L, Cordewener J, America T, Gallerani R, Bode W . Properties of purified gut trypsin from Helicoverpa zea, adapted to proteinase inhibitors. Eur J Biochem. 2002; 270(1):10-9. DOI: 10.1046/j.1432-1033.2003.03368.x. View

Thompson J, Perry K, de Jong W . A new potato virus in a new lineage of picorna-like viruses. Arch Virol. 2004; 149(11):2141-54. DOI: 10.1007/s00705-004-0362-3. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Bazan J, Fletterick R . Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implications. Proc Natl Acad Sci U S A. 1988; 85(21):7872-6. PMC: 282299. DOI: 10.1073/pnas.85.21.7872. View

Argos P . A sequence motif in many polymerases. Nucleic Acids Res. 1988; 16(21):9909-16. PMC: 338826. DOI: 10.1093/nar/16.21.9909. View

Gorbalenya A, Koonin E, Wolf Y . A new superfamily of putative NTP-binding domains encoded by genomes of small DNA and RNA viruses. FEBS Lett. 1990; 262(1):145-8. DOI: 10.1016/0014-5793(90)80175-i. View

Koonin E . The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J Gen Virol. 1991; 72 ( Pt 9):2197-206. DOI: 10.1099/0022-1317-72-9-2197. View

10.

Mushegian A . The putative movement domain encoded by nepovirus RNA-2 is conserved in all sequenced nepoviruses. Arch Virol. 1994; 135(3-4):437-41. DOI: 10.1007/BF01310028. View

11.

Shevchenko A, Wilm M, Vorm O, Mann M . Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem. 1996; 68(5):850-8. DOI: 10.1021/ac950914h. View

12.

Page R . TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci. 1996; 12(4):357-8. DOI: 10.1093/bioinformatics/12.4.357. View

13.

Thompson J, Gibson T, Plewniak F, Jeanmougin F, Higgins D . The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1998; 25(24):4876-82. PMC: 147148. DOI: 10.1093/nar/25.24.4876. View

14.

James D, Upton C . Genome segment RNA-1 of a flat apple isolate of Cherry rasp leaf virus: nucleotide sequence analysis and RT-PCR detection. Arch Virol. 2005; 150(7):1469-76. DOI: 10.1007/s00705-005-0503-3. View

15.

James D, Upton C . Nucleotide sequence analysis of RNA-2 of a flat apple isolate of Cherry rasp leaf virus with regions showing greater identity to animal picornaviruses than to related plant viruses. Arch Virol. 2002; 147(8):1631-41. DOI: 10.1007/s00705-002-0833-3. View