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Detection of Helicobacter Pylori in Stomach Tissue by Use of a Monoclonal Antibody

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1991 Dec 1
PMID 1721923
Citations 5
Authors
M O Husson
H Leclerc
Affiliations
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Abstract

Monoclonal antibodies were produced against an acid glycine extract of Helicobacter pylori ATCC 43504T. One of these appeared to be specific for H. pylori; it recognized all H. pylori isolates by an indirect immunofluorescence assay (IIF) but it did not cross-react with the other strains tested, including different species of the genera Helicobacter, Campylobacter, and Wolinella. Different strains of members of the families Enterobacteriaceae and Pseudomonadaceae or other gram-negative bacteria tested also gave negative reactions. Indirect immunofluorescence assay of antral biopsy specimens identified 54 of 56 infected patients (96.4%), and it may be able to detect nonviable organisms after antibiotic therapy.

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References
1.
Westblom T, Madan E, Kemp J, Subik M . Evaluation of a rapid urease test to detect Campylobacter pylori infection. J Clin Microbiol. 1988; 26(7):1393-4. PMC: 266616. DOI: 10.1128/jcm.26.7.1393-1394.1988. View
2.
Morris A, Ali M, Brown P, Lane M, Patton K . Campylobacter pylori infection in biopsy specimens of gastric antrum: laboratory diagnosis and estimation of sampling error. J Clin Pathol. 1989; 42(7):727-32. PMC: 1142023. DOI: 10.1136/jcp.42.7.727. View
3.
Taylor D, Hargreaves J, Ng L, SHERBANIUK R, Jewell L . Isolation and characterization of Campylobacter pyloridis from gastric biopsies. Am J Clin Pathol. 1987; 87(1):49-54. DOI: 10.1093/ajcp/87.1.49. View
4.
Engstrand L, Pahlson C, Gustavsson S, Schwan A . Monoclonal antibodies for rapid identification of Campylobacter pyloridis. Lancet. 1986; 2(8520):1402-3. DOI: 10.1016/s0140-6736(86)92049-0. View
5.
Negrini R, Lisato L, Cavazzini L, Maini P, Gullini S, Basso O . Monoclonal antibodies for specific immunoperoxidase detection of Campylobacter pylori. Gastroenterology. 1989; 96(2 Pt 1):414-20. DOI: 10.1016/0016-5085(89)91565-5. View