ZAP-70 Kinase Regulates HIV Cell-to-cell Spread and Virological Synapse Formation

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2007 Jan 12

PMID 17215865

Citations 77

Authors

Nathalie Sol-Foulon

Marion Sourisseau

Francoise Porrot

Maria-Isabel Thoulouze

Celine Trouillet

Cinzia Nobile

Fabien Blanchet

Vincenzo Di Bartolo

Nelly Noraz

Naomi Taylor

Andres Alcover

Claire Hivroz

Olivier Schwartz

Affiliations

Soon will be listed here.

Abstract

HIV efficiently spreads in lymphocytes, likely through virological synapses (VSs). These cell-cell junctions share some characteristics with immunological synapses, but cellular proteins required for their constitution remain poorly characterized. We have examined here the role of ZAP-70, a key kinase regulating T-cell activation and immunological synapse formation, in HIV replication. In lymphocytes deficient for ZAP-70, or expressing a kinase-dead mutant of the protein, HIV replication was strikingly delayed. We have characterized further this replication defect. ZAP-70 was dispensable for the early steps of viral cycle, from entry to expression of viral proteins. However, in the absence of ZAP-70, intracellular Gag localization was impaired. ZAP-70 was required in infected donor cells for efficient cell-to-cell HIV transmission to recipients and for formation of VSs. These results bring novel insights into the links that exist between T-cell activation and HIV spread, and suggest that HIV usurps components of the immunological synapse machinery to ensure its own spread through cell-to-cell contacts.

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