Assignment of the Ferriheme Resonances of the High-spin Forms of Nitrophorins 1 and 4 by 1H NMR Spectroscopy: Comparison to Structural Data Obtained from X-ray Crystallography

Overview

Journal Inorg Chem

Specialty Chemistry

Date 2007 Jan 3

PMID 17198425

Citations 13

Authors

Tatiana Kh Shokhireva

Kevin M Smith

Robert E Berry

Nikolai V Shokhirev

Celia A Balfour

Hongjun Zhang

F Ann Walker

Affiliations

Soon will be listed here.

Abstract

In this work, we report the assignment of the majority of the ferriheme resonances of high-spin nitrophorins (NPs) 1 and 4 and compare them to those of NP2, published previously. It is found that the structures of the ferriheme complexes of NP1 and NP4, in terms of the orientation of the histidine imidazole ligand, can be described with good accuracy by NMR techniques and that the angle plot proposed previously for the high-spin form of the NPs (Shokhireva, T. Kh.; Shokhirev, N. V.; Walker, F. A. Biochemistry 2003, 42, 679-693) describes the angle of the effective nodal plane of the axial histidine imidazole in solution. There is an equilibrium between the two heme orientations (A and B), which depends on the heme cavity shape, which can be altered by mutation of amino acids with side chains (phenyl vs tyrosyl) near the potential position where a heme vinyl group would be in one of the isomers. The A:B ratio can be much more accurately measured by NMR spectroscopy than by X-ray crystallography.

Citing Articles

Dimerization of nitrophorin 4 at low pH and comparison to the K1A mutant of nitrophorin 1.

Berry R, Yang F, Shokhireva T, Amoia A, Garrett S, Goren A Biochemistry. 2014; 54(2):208-20.

PMID: 25489673 PMC: 4303305. DOI: 10.1021/bi5013047.

NMR investigations of nitrophorin 2 belt side chain effects on heme orientation and seating of native N-terminus NP2 and NP2(D1A).

Berry R, Muthu D, Shokhireva T, Garrett S, Goren A, Zhang H J Biol Inorg Chem. 2013; 19(4-5):577-93.

PMID: 24292244 PMC: 4031258. DOI: 10.1007/s00775-013-1063-8.

Electron spin density on the axial His ligand of high-spin and low-spin nitrophorin 2 probed by heteronuclear NMR spectroscopy.

Abriata L, Zaballa M, Berry R, Yang F, Zhang H, Walker F Inorg Chem. 2013; 52(3):1285-95.

PMID: 23327568 PMC: 3594510. DOI: 10.1021/ic301805y.

Native N-terminus nitrophorin 2 from the kissing bug: similarities to and differences from NP2(D1A).

Berry R, Muthu D, Shokhireva T, Garrett S, Zhang H, Walker F Chem Biodivers. 2012; 9(9):1739-55.

PMID: 22976966 PMC: 3523740. DOI: 10.1002/cbdv.201100449.

Assignment of the 1H NMR resonances of protein residues in close proximity to the heme of the nitrophorins: similarities and differences among the four proteins from the saliva of the adult blood-sucking insect Rhodnius prolixus.

Shokhireva T, Walker F J Biol Inorg Chem. 2012; 17(6):911-26.

PMID: 22711329 DOI: 10.1007/s00775-012-0908-x.

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