Bacterial Community Structure of Biofilms on Artificial Surfaces in an Estuary

Overview

Journal Microb Ecol

Specialties Environmental Health
Microbiology

Date 2006 Dec 23

PMID 17186146

Citations 40

Authors

Paul R Jones

Matthew T Cottrell

David L Kirchman

Stephen C Dexter

Affiliations

Soon will be listed here.

Abstract

This study examined bacterial community structure of biofilms on stainless steel and polycarbonate in seawater from the Delaware Bay. Free-living bacteria in the surrounding seawater were compared to the attached bacteria during the first few weeks of biofilm growth. Surfaces exposed to seawater were analyzed by using 16S rDNA libraries, fluorescence in situ hybridization (FISH), and denaturing gradient gel electrophoresis (DGGE). Community structure of the free-living bacterial community was different from that of the attached bacteria according to FISH and DGGE. In particular, alpha-proteobacteria dominated the attached communities. Libraries of 16S rRNA genes revealed that representatives of the Rhodobacterales clade were the most abundant members of biofilm communities. Changes in community structure during biofilm growth were also examined by DGGE analysis. We hypothesized that bacterial communities on dissimilar surfaces would initially differ and become more similar over time. In contrast, the compositions of stainless steel and polycarbonate biofilms were initially the same, but differed after about 1 week of biofilm growth. These data suggest that the relationship between surface properties and biofilm community structure changes as biofilms grow on surfaces such as stainless steel and polycarbonate in estuarine water.

Citing Articles

Marine Bacterial Community Structures of Selected Coastal Seawater and Sediment Sites in Qatar.

El-Malah S, Rasool K, Jabbar K, Sohail M, Baalousha H, Mahmoud K Microorganisms. 2023; 11(12).

PMID: 38137970 PMC: 10745943. DOI: 10.3390/microorganisms11122827.

Microbiologically influenced corrosion of AISI 202 and 316L stainless steels under manganese-oxidizing biofilms.

Balakrishnan A, Dhaipule N, Philip J 3 Biotech. 2023; 14(1):12.

PMID: 38107030 PMC: 10719233. DOI: 10.1007/s13205-023-03845-z.

Micron-Scale Biogeography of Seawater Biofilm Colonies at Submersed Solid Substrata Affected by Organic Matter and Microbiome Transformation in the Baltic Sea.

Grzegorczyk M, Pogorzelski S, Janowicz P, Boniewicz-Szmyt K, Rochowski P Materials (Basel). 2022; 15(18).

PMID: 36143678 PMC: 9501339. DOI: 10.3390/ma15186351.

Marine biofilms: diversity, interactions and biofouling.

Qian P, Cheng A, Wang R, Zhang R Nat Rev Microbiol. 2022; 20(11):671-684.

PMID: 35614346 DOI: 10.1038/s41579-022-00744-7.

Impacts of UV-C Irradiation on Marine Biofilm Community Succession.

Naik A, Smithers M, Moisander P Appl Environ Microbiol. 2021; 88(4):e0229821.

PMID: 34936837 PMC: 8863041. DOI: 10.1128/aem.02298-21.

References

Dang H, Lovell C . Bacterial primary colonization and early succession on surfaces in marine waters as determined by amplified rRNA gene restriction analysis and sequence analysis of 16S rRNA genes. Appl Environ Microbiol. 2000; 66(2):467-75. PMC: 91850. DOI: 10.1128/AEM.66.2.467-475.2000. View

Araya R, Tani K, Takagi T, Yamaguchi N, Nasu M . Bacterial activity and community composition in stream water and biofilm from an urban river determined by fluorescent in situ hybridization and DGGE analysis. FEMS Microbiol Ecol. 2009; 43(1):111-9. DOI: 10.1111/j.1574-6941.2003.tb01050.x. View

Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhukumar . ARB: a software environment for sequence data. Nucleic Acids Res. 2004; 32(4):1363-71. PMC: 390282. DOI: 10.1093/nar/gkh293. View

Brummer I, FEHR W, Wagner-Dobler I . Biofilm community structure in polluted rivers: abundance of dominant phylogenetic groups over a complete annual cycle. Appl Environ Microbiol. 2000; 66(7):3078-82. PMC: 92114. DOI: 10.1128/AEM.66.7.3078-3082.2000. View

Videla H, Herrera L . Microbiologically influenced corrosion: looking to the future. Int Microbiol. 2005; 8(3):169-80. View

Francis C, Co E, Tebo B . Enzymatic manganese(II) oxidation by a marine alpha-proteobacterium. Appl Environ Microbiol. 2001; 67(9):4024-9. PMC: 93124. DOI: 10.1128/AEM.67.9.4024-4029.2001. View

Cottrell M, Kirchman D . Natural assemblages of marine proteobacteria and members of the Cytophaga-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Appl Environ Microbiol. 2000; 66(4):1692-7. PMC: 92043. DOI: 10.1128/AEM.66.4.1692-1697.2000. View

Bhosle N, Garg A, Fernandes L, Citon P . Dynamics of amino acids in the conditioning film developed on glass panels immersed in the surface seawaters of Dona Paula Bay. Biofouling. 2005; 21(2):99-107. DOI: 10.1080/08927010500097821. View

Giovannoni S, Britschgi T, Moyer C, Field K . Genetic diversity in Sargasso Sea bacterioplankton. Nature. 1990; 345(6270):60-3. DOI: 10.1038/345060a0. View

10.

Glockner F, Fuchs B, Amann R . Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol. 1999; 65(8):3721-6. PMC: 91558. DOI: 10.1128/AEM.65.8.3721-3726.1999. View

11.

Cottrell M, Kirchman D . Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl Environ Microbiol. 2000; 66(12):5116-22. PMC: 92431. DOI: 10.1128/AEM.66.12.5116-5122.2000. View

12.

Weller R, Glockner F, Amann R . 16S rRNA-targeted oligonucleotide probes for the in situ detection of members of the phylum Cytophaga-Flavobacterium-Bacteroides. Syst Appl Microbiol. 2000; 23(1):107-14. DOI: 10.1016/S0723-2020(00)80051-X. View

13.

Amann R, Krumholz L, Stahl D . Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol. 1990; 172(2):762-70. PMC: 208504. DOI: 10.1128/jb.172.2.762-770.1990. View

14.

Dang H, Lovell C . Numerical dominance and phylotype diversity of marine Rhodobacter species during early colonization of submerged surfaces in coastal marine waters as determined by 16S ribosomal DNA sequence analysis and fluorescence in situ hybridization. Appl Environ Microbiol. 2002; 68(2):496-504. PMC: 126732. DOI: 10.1128/AEM.68.2.496-504.2002. View

15.

Bruhn J, Nielsen K, Hjelm M, Hansen M, Bresciani J, Schulz S . Ecology, inhibitory activity, and morphogenesis of a marine antagonistic bacterium belonging to the Roseobacter clade. Appl Environ Microbiol. 2005; 71(11):7263-70. PMC: 1287604. DOI: 10.1128/AEM.71.11.7263-7270.2005. View

16.

Crump B, Armbrust E, Baross J . Phylogenetic analysis of particle-attached and free-living bacterial communities in the Columbia river, its estuary, and the adjacent coastal ocean. Appl Environ Microbiol. 1999; 65(7):3192-204. PMC: 91474. DOI: 10.1128/AEM.65.7.3192-3204.1999. View

17.

Bidle K, Fletcher M . Comparison of free-living and particle-associated bacterial communities in the chesapeake bay by stable low-molecular-weight RNA analysis. Appl Environ Microbiol. 1995; 61(3):944-52. PMC: 1388377. DOI: 10.1128/aem.61.3.944-952.1995. View

18.

Karner M, Fuhrman J . Determination of Active Marine Bacterioplankton: a Comparison of Universal 16S rRNA Probes, Autoradiography, and Nucleoid Staining. Appl Environ Microbiol. 1997; 63(4):1208-13. PMC: 1389541. DOI: 10.1128/aem.63.4.1208-1213.1997. View

19.

Fuchs B, Wallner G, Beisker W, Schwippl I, Ludwig W, Amann R . Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes. Appl Environ Microbiol. 1998; 64(12):4973-82. PMC: 90951. DOI: 10.1128/AEM.64.12.4973-4982.1998. View

20.

Suzuki M, Giovannoni S . Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR. Appl Environ Microbiol. 1996; 62(2):625-30. PMC: 167828. DOI: 10.1128/aem.62.2.625-630.1996. View