Coexpression of Alpha 1 with Putative Beta 3 Subunits Results in Functional Na+/K+ Pumps in Xenopus Oocytes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1991 Oct 1

PMID 1717977

Citations 18

Authors

J D Horisberger

P Jaunin

P J Good

B C Rossier

K Geering

Affiliations

Soon will be listed here.

Abstract

The active Na+/K+ pump is composed of an alpha and a beta subunit. Until now, three putative isoforms of the beta subunit have been identified that share sequence similarity. We have expressed the beta 1 and beta 3 isoforms of Xenopus laevis Na+/K(+)-ATPase in Xenopus oocytes to compare functional properties of the Na+/K+ pump, including either of these two isoforms. Na+/K+ pump current, estimated as K(+)-induced outward current in voltage-clamped oocytes, was doubled by coexpression of alpha 1 subunits with either isoform of the beta subunit compared to expression of alpha 1 subunits alone. The kinetics of activation by external K+ and the voltage dependence of the electrogenic activity of the Na+/K+ pump were similar with both beta isoforms, indicating that both beta 1 and beta 3 isoforms can support expression at the oocyte surface of an active Na+/K+ pump with similar functional properties.

Citing Articles

Endogenous cardiotonic steroids and cardiovascular disease, where to next?.

Pavlovic D Cell Calcium. 2020; 86:102156.

PMID: 31896530 PMC: 7031694. DOI: 10.1016/j.ceca.2019.102156.

Clock-Generated Temporal Codes Determine Synaptic Plasticity to Control Sleep.

Tabuchi M, Monaco J, Duan G, Bell B, Liu S, Liu Q Cell. 2018; 175(5):1213-1227.e18.

PMID: 30318147 PMC: 6239908. DOI: 10.1016/j.cell.2018.09.016.

The inner mantle of the giant clam, Tridacna squamosa, expresses a basolateral Na+/K+-ATPase α-subunit, which displays light-dependent gene and protein expression along the shell-facing epithelium.

Boo M, Hiong K, Choo C, Cao-Pham A, Wong W, Chew S PLoS One. 2017; 12(10):e0186865.

PMID: 29049367 PMC: 5648256. DOI: 10.1371/journal.pone.0186865.

Mineralocorticoids modulate the expression of the β-3 subunit of the Na, K-ATPase in the renal collecting duct.

Rojas M, Diaz P, Leon P, Gonzalez A, Gonzalez M, Barrientos V Channels (Austin). 2017; 11(5):388-398.

PMID: 28636485 PMC: 5626372. DOI: 10.1080/19336950.2017.1344800.

Regulation of the cardiac sodium pump.

Fuller W, Tulloch L, Shattock M, Calaghan S, Howie J, Wypijewski K Cell Mol Life Sci. 2012; 70(8):1357-80.

PMID: 22955490 PMC: 3607738. DOI: 10.1007/s00018-012-1134-y.

References

Good P, Richter K, Dawid I . A nervous system-specific isotype of the beta subunit of Na+,K(+)-ATPase expressed during early development of Xenopus laevis. Proc Natl Acad Sci U S A. 1990; 87(23):9088-92. PMC: 55109. DOI: 10.1073/pnas.87.23.9088. View

Latorre R, Miller C . Conduction and selectivity in potassium channels. J Membr Biol. 1983; 71(1-2):11-30. DOI: 10.1007/BF01870671. View

Rakowski R, Vasilets L, Latona J, Schwarz W . A negative slope in the current-voltage relationship of the Na+/K+ pump in Xenopus oocytes produced by reduction of external [K+]. J Membr Biol. 1991; 121(2):177-87. DOI: 10.1007/BF01870531. View

Good P, Welch R, Barkan A, Somasekhar M, Mertz J . Both VP2 and VP3 are synthesized from each of the alternative spliced late 19S RNA species of simian virus 40. J Virol. 1988; 62(3):944-53. PMC: 253653. DOI: 10.1128/JVI.62.3.944-953.1988. View

Noguchi S, Mishina M, Kawamura M, Numa S . Expression of functional (Na+ + K+)-ATPase from cloned cDNAs. FEBS Lett. 1987; 225(1-2):27-32. DOI: 10.1016/0014-5793(87)81125-0. View

Schwarz W, Gu Q . Characteristics of the Na+/K+-ATPase from Torpedo californica expressed in Xenopus oocytes: a combination of tracer flux measurements with electrophysiological measurements. Biochim Biophys Acta. 1988; 945(2):167-74. DOI: 10.1016/0005-2736(88)90479-8. View

Lauger P . Dynamics of ion transport systems in membranes. Physiol Rev. 1987; 67(4):1296-331. DOI: 10.1152/physrev.1987.67.4.1296. View

Apell H . Electrogenic properties of the Na,K pump. J Membr Biol. 1989; 110(2):103-14. DOI: 10.1007/BF01869466. View

Geering K, Theulaz I, Verrey F, Hauptle M, Rossier B . A role for the beta-subunit in the expression of functional Na+-K+-ATPase in Xenopus oocytes. Am J Physiol. 1989; 257(5 Pt 1):C851-8. DOI: 10.1152/ajpcell.1989.257.5.C851. View

10.

Martin-Vasallo P, Dackowski W, Emanuel J, Levenson R . Identification of a putative isoform of the Na,K-ATPase beta subunit. Primary structure and tissue-specific expression. J Biol Chem. 1989; 264(8):4613-8. View

11.

Verrey F, Kairouz P, SCHAERER E, Fuentes P, Geering K, Rossier B . Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells. Am J Physiol. 1989; 256(6 Pt 2):F1034-43. DOI: 10.1152/ajprenal.1989.256.6.F1034. View

12.

Gadsby D, Nakao M . Steady-state current-voltage relationship of the Na/K pump in guinea pig ventricular myocytes. J Gen Physiol. 1989; 94(3):511-37. PMC: 2228955. DOI: 10.1085/jgp.94.3.511. View

13.

Horisberger J, Giebisch G . Na-K pump current in the Amphiuma collecting tubule. J Gen Physiol. 1989; 94(3):493-510. PMC: 2228957. DOI: 10.1085/jgp.94.3.493. View

14.

Rakowski R, Paxson C . Voltage dependence of Na/K pump current in Xenopus oocytes. J Membr Biol. 1988; 106(2):173-82. DOI: 10.1007/BF01871399. View

15.

Richter K, Grunz H, Dawid I . Gene expression in the embryonic nervous system of Xenopus laevis. Proc Natl Acad Sci U S A. 1988; 85(21):8086-90. PMC: 282359. DOI: 10.1073/pnas.85.21.8086. View

16.

Melton D, Krieg P, Rebagliati M, Maniatis T, Zinn K, Green M . Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984; 12(18):7035-56. PMC: 320141. DOI: 10.1093/nar/12.18.7035. View

17.

Gloor S, Antonicek H, Sweadner K, Pagliusi S, Frank R, Moos M . The adhesion molecule on glia (AMOG) is a homologue of the beta subunit of the Na,K-ATPase. J Cell Biol. 1990; 110(1):165-74. PMC: 2115981. DOI: 10.1083/jcb.110.1.165. View

18.

Ackermann U, Geering K . Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport. FEBS Lett. 1990; 269(1):105-8. DOI: 10.1016/0014-5793(90)81130-g. View

19.

Geering K . Subunit assembly and functional maturation of Na,K-ATPase. J Membr Biol. 1990; 115(2):109-21. DOI: 10.1007/BF01869450. View

20.

Shyjan A, Gottardi C, Levenson R . The Na,K-ATPase beta 2 subunit is expressed in rat brain and copurifies with Na,K-ATPase activity. J Biol Chem. 1990; 265(9):5166-9. View