AGP/EBP(LAP) Expressed in Rat Hepatoma Cells Interacts with Multiple Promoter Sites and is Necessary for Maximal Glucocorticoid Induction of the Rat Alpha-1 Acid Glycoprotein Gene

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1991 Oct 1

PMID 1717823

Citations 11

Authors

P Williams

T Ratajczak

S C Lee

G M Ringold

Affiliations

Soon will be listed here.

Abstract

Transcription of the rat alpha 1-acid glycoprotein (AGP) gene is induced by glucocorticoids. In addition to the glucocorticoid response element which maps to bases -120 to -107, sequences located between bases -106 to -42 have been shown to be necessary for hormone induction. We have previously identified multiple sites of C/EBP interaction with the AGP promoter in the region -106 to -64. In this study, we purify and identify a C/EBP family member, AGP/EBP(LAP), present in the rat hepatoma cell line HTC (JZ.1) which also binds to the C/EBP recognition sites in this region. Mutations in the recognition sites that prevent binding are analyzed, and the results suggest a positive as well as a possible inhibitory role for AGP/EBP(LAP) in the glucocorticoid induction of the gene in HTC (JZ.1) cells.

Citing Articles

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Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression.

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