DNA Polymorphism in Strains of Listeria Monocytogenes

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1991 Jul 1

PMID 1715882

Citations 17

Authors

C Carriere

A Allardet-Servent

G Bourg

A Audurier

M Ramuz

Affiliations

Soon will be listed here.

Abstract

DNA polymorphism in 35 Listeria monocytogenes strains belonging to serovars 1/2a, 1/2b, 1/2c, and 4b was studied by genomic DNA digestion. The restriction endonucleases ApaI and NotI, which cleave DNA at rare sequences, were used, and DNA fragments were analyzed by pulsed-field gel electrophoresis. Restriction fragment length polymorphism varied among different serovars and was used for epidemiological studies, but serovar 1/2c isolates could not be analyzed because their restriction patterns were indistinguishable. The genome sizes were calculated by addition of the sizes of the ApaI fragments and were found to be about 2,660 kb for serovar 1/2a strains, 2,640 kb for serovar 1/2b strains, and 2,710 kb for serovar 4b strains but only 2,340 kb for serovar 1/2c strains. This last group therefore appears to differ from the other serovar strains by the absence of restriction fragment length polymorphism and a chromosome that is 15% shorter, suggesting that strains of serovar 1/2c have quite recently emerged.

Citing Articles

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Oh H, Kim S, Lee S, Lee H, Ha J, Lee J Korean J Food Sci Anim Resour. 2018; 38(5):851-865.

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It Is Not All about Single Nucleotide Polymorphisms: Comparison of Mobile Genetic Elements and Deletions in Listeria monocytogenes Genomes Links Cases of Hospital-Acquired Listeriosis to the Environmental Source.

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Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR.

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Genetic typing of human and food isolates of Listeria monocytogenes from episodes of listeriosis.

Franciosa G, Pourshaban M, Gianfranceschi M, Aureli P Eur J Epidemiol. 1998; 14(2):205-10.

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