Sequence and Properties of ComQ, a New Competence Regulatory Gene of Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Sep 1

PMID 1715859

Citations 47

Authors

Y Weinrauch

T Msadek

F Kunst

D Dubnau

Affiliations

Soon will be listed here.

Abstract

The sequence and properties of the comQ gene are described. comQ was predicted to encode a 34,209-Da protein, and the product of comQ was shown to be required for the development of genetic competence. The apparent transcriptional initiation and termination sites of comQ were mapped, and the location of a likely E sigma A promoter was inferred. The expression of comQ was maximal early in growth and declined as the cells approached the stationary phase. This expression was not dependent on any of the competence regulatory genes tested (comA, comP, sin, abrB, degU, and spo0A). Disruption of comQ in the chromosome prevented the development of competence as well as the transcription of comG, a late competence operon. This disruption also decreased the expression of srfA, a regulatory operon needed for the expression of competence. These and other results suggest a role for ComQ early in the hierarchy of competence regulatory genes, probably as a component of a signal transduction system.

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References

Mukai K, Kawata M, Tanaka T . Isolation and phosphorylation of the Bacillus subtilis degS and degU gene products. J Biol Chem. 1990; 265(32):20000-6. View

Perego M, Spiegelman G, Hoch J . Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis. Mol Microbiol. 1988; 2(6):689-99. DOI: 10.1111/j.1365-2958.1988.tb00079.x. View

Burbulys D, Trach K, Hoch J . Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay. Cell. 1991; 64(3):545-52. DOI: 10.1016/0092-8674(91)90238-t. View

Dahl M, Msadek T, Kunst F, Rapoport G . Mutational analysis of the Bacillus subtilis DegU regulator and its phosphorylation by the DegS protein kinase. J Bacteriol. 1991; 173(8):2539-47. PMC: 207818. DOI: 10.1128/jb.173.8.2539-2547.1991. View

Monod M, Denoya C, Dubnau D . Sequence and properties of pIM13, a macrolide-lincosamide-streptogramin B resistance plasmid from Bacillus subtilis. J Bacteriol. 1986; 167(1):138-47. PMC: 212852. DOI: 10.1128/jb.167.1.138-147.1986. View

Amory A, Kunst F, Aubert E, Klier A, Rapoport G . Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis. J Bacteriol. 1987; 169(1):324-33. PMC: 211771. DOI: 10.1128/jb.169.1.324-333.1987. View

Denoya C, Bechhofer D, Dubnau D . Translational autoregulation of ermC 23S rRNA methyltransferase expression in Bacillus subtilis. J Bacteriol. 1986; 168(3):1133-41. PMC: 213613. DOI: 10.1128/jb.168.3.1133-1141.1986. View

Albano M, Hahn J, Dubnau D . Expression of competence genes in Bacillus subtilis. J Bacteriol. 1987; 169(7):3110-7. PMC: 212357. DOI: 10.1128/jb.169.7.3110-3117.1987. View

Villafane R, Bechhofer D, Narayanan C, Dubnau D . Replication control genes of plasmid pE194. J Bacteriol. 1987; 169(10):4822-9. PMC: 213861. DOI: 10.1128/jb.169.10.4822-4829.1987. View

10.

Stock J, Ninfa A, Stock A . Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev. 1989; 53(4):450-90. PMC: 372749. DOI: 10.1128/mr.53.4.450-490.1989. View

11.

Weinrauch Y, Guillen N, Dubnau D . Sequence and transcription mapping of Bacillus subtilis competence genes comB and comA, one of which is related to a family of bacterial regulatory determinants. J Bacteriol. 1989; 171(10):5362-75. PMC: 210374. DOI: 10.1128/jb.171.10.5362-5375.1989. View

12.

Guillen N, Weinrauch Y, Dubnau D . Cloning and characterization of the regulatory Bacillus subtilis competence genes comA and comB. J Bacteriol. 1989; 171(10):5354-61. PMC: 210373. DOI: 10.1128/jb.171.10.5354-5361.1989. View

13.

Nakano M, Zuber P . Cloning and characterization of srfB, a regulatory gene involved in surfactin production and competence in Bacillus subtilis. J Bacteriol. 1989; 171(10):5347-53. PMC: 210372. DOI: 10.1128/jb.171.10.5347-5353.1989. View

14.

Jaacks K, Healy J, Losick R, Grossman A . Identification and characterization of genes controlled by the sporulation-regulatory gene spo0H in Bacillus subtilis. J Bacteriol. 1989; 171(8):4121-9. PMC: 210181. DOI: 10.1128/jb.171.8.4121-4129.1989. View

15.

Engelman D, Steitz T, Goldman A . Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins. Annu Rev Biophys Biophys Chem. 1986; 15:321-53. DOI: 10.1146/annurev.bb.15.060186.001541. View

16.

Ulmanen I, Lundstrom K, Lehtovaara P, Sarvas M, Ruohonen M, PALVA I . Transcription and translation of foreign genes in Bacillus subtilis by the aid of a secretion vector. J Bacteriol. 1985; 162(1):176-82. PMC: 218971. DOI: 10.1128/jb.162.1.176-182.1985. View

17.

Msadek T, Kunst F, Henner D, Klier A, Rapoport G, DEDONDER R . Signal transduction pathway controlling synthesis of a class of degradative enzymes in Bacillus subtilis: expression of the regulatory genes and analysis of mutations in degS and degU. J Bacteriol. 1990; 172(2):824-34. PMC: 208512. DOI: 10.1128/jb.172.2.824-834.1990. View

18.

Weinrauch Y, Penchev R, DUBNAU E, Smith I, Dubnau D . A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems. Genes Dev. 1990; 4(5):860-72. DOI: 10.1101/gad.4.5.860. View

19.

Roggiani M, Hahn J, Dubnau D . Suppression of early competence mutations in Bacillus subtilis by mec mutations. J Bacteriol. 1990; 172(7):4056-63. PMC: 213392. DOI: 10.1128/jb.172.7.4056-4063.1990. View

20.

Gryczan T, Dubnau D . Induction of macrolide-lincosamide-streptogramin B resistance requires ribosomes able to bind inducer. Mol Gen Genet. 1984; 194(3):357-61. DOI: 10.1007/BF00425544. View