Liquid Junction Potentials and Small Cell Effects in Patch-clamp Analysis

Overview

Journal J Membr Biol

Date 1991 Apr 1

PMID 1715403

Citations 278

Authors

P H Barry

J W Lynch

Affiliations

Soon will be listed here.

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References

Hamill O, Marty A, Neher E, Sakmann B, Sigworth F . Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981; 391(2):85-100. DOI: 10.1007/BF00656997. View

Cahalan M, Chandy K, DeCoursey T, Gupta S . A voltage-gated potassium channel in human T lymphocytes. J Physiol. 1985; 358:197-237. PMC: 1193339. DOI: 10.1113/jphysiol.1985.sp015548. View

Schild D . Whole-cell currents in olfactory receptor cells of Xenopus laevis. Exp Brain Res. 1989; 78(2):223-32. DOI: 10.1007/BF00228894. View

Lynch J, Barry P . Action potentials initiated by single channels opening in a small neuron (rat olfactory receptor). Biophys J. 1989; 55(4):755-68. PMC: 1330559. DOI: 10.1016/S0006-3495(89)82874-7. View

Trotier D . A patch-clamp analysis of membrane currents in salamander olfactory receptor cells. Pflugers Arch. 1986; 407(6):589-95. DOI: 10.1007/BF00582636. View

Fernandez J, Fox A, Krasne S . Membrane patches and whole-cell membranes: a comparison of electrical properties in rat clonal pituitary (GH3) cells. J Physiol. 1984; 356:565-85. PMC: 1193182. DOI: 10.1113/jphysiol.1984.sp015483. View

Lynch J, Barry P . Properties of transient K+ currents and underlying single K+ channels in rat olfactory receptor neurons. J Gen Physiol. 1991; 97(5):1043-72. PMC: 2216501. DOI: 10.1085/jgp.97.5.1043. View

Firestein S, Werblin F . Gated currents in isolated olfactory receptor neurons of the larval tiger salamander. Proc Natl Acad Sci U S A. 1987; 84(17):6292-6. PMC: 299057. DOI: 10.1073/pnas.84.17.6292. View

Deutsch C, Krause D, Lee S . Voltage-gated potassium conductance in human T lymphocytes stimulated with phorbol ester. J Physiol. 1986; 372:405-23. PMC: 1192770. DOI: 10.1113/jphysiol.1986.sp016016. View

10.

Kehl S, McBurney R . The firing patterns of rat melanotrophs recorded using the patch clamp technique. Neuroscience. 1989; 33(3):579-86. DOI: 10.1016/0306-4522(89)90410-7. View

11.

CALDWELL P . Liquid junction potentials and their effect on potential measurements in biological systems. Int Rev Cytol. 1968; 24:345-71. DOI: 10.1016/s0074-7696(08)61402-3. View

12.

Barry P, Diamond J . Junction potentials, electrode standard potentials, and other problems in interpreting electrical properties of membranes. J Membr Biol. 2013; 3(1):93-122. DOI: 10.1007/BF01868010. View

13.

Neher E, Sakmann B, Steinbach J . The extracellular patch clamp: a method for resolving currents through individual open channels in biological membranes. Pflugers Arch. 1978; 375(2):219-28. DOI: 10.1007/BF00584247. View

14.

Fischmeister R, Ayer Jr R, Dehaan R . Some limitations of the cell-attached patch clamp technique: a two-electrode analysis. Pflugers Arch. 1986; 406(1):73-82. DOI: 10.1007/BF00582957. View

15.

Atwater I, Rosario L, Rojas E . Properties of the Ca-activated K+ channel in pancreatic beta-cells. Cell Calcium. 1983; 4(5-6):451-61. DOI: 10.1016/0143-4160(83)90021-0. View

16.

Frings S, Lindemann B . Odorant response of isolated olfactory receptor cells is blocked by amiloride. J Membr Biol. 1988; 105(3):233-43. DOI: 10.1007/BF01871000. View

17.

Pusch M, Neher E . Rates of diffusional exchange between small cells and a measuring patch pipette. Pflugers Arch. 1988; 411(2):204-11. DOI: 10.1007/BF00582316. View

18.

Maue R, Dionne V . Patch-clamp studies of isolated mouse olfactory receptor neurons. J Gen Physiol. 1987; 90(1):95-125. PMC: 2228861. DOI: 10.1085/jgp.90.1.95. View

19.

Fenwick E, Marty A, Neher E . A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine. J Physiol. 1982; 331:577-97. PMC: 1197770. DOI: 10.1113/jphysiol.1982.sp014393. View

20.

Barry P . Ionic permeation mechanisms in epithelia: biionic potentials, dilution potentials, conductances, and streaming potentials. Methods Enzymol. 1989; 171:678-715. DOI: 10.1016/s0076-6879(89)71038-7. View