Astroglial-conditioned Media and Growth Factors Modulate Proliferation and Differentiation of Astrocytes in Primary Culture

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 2006 Dec 8

PMID 17151914

Citations 5

Authors

Vincenzo Bramanti

Agata Campisi

Daniele Tomassoni

Antonino Costa

Alfredo Fisichella

Venera Mazzone

Luca Denaro

Marcello Avitabile

Francesco Amenta

Roberto Avola

Affiliations

Soon will be listed here.

Abstract

Astroglial conditioned media (ACM) influence the development and maturation of cultured nerve cells and modulate neuron-glia interaction. To clarify mechanisms of astroglial cell proliferation/differentiation in culture, incorporation of [methyl-3H]-thymidine or [5,6-3H]-uridine in cultured astrocytes was assessed. Cultures were pre-treated with epidermal growth factor (EGF), insulin (INS), insulin-like growth factor-I (IGF-I), and basic fibroblast growth factor (bFGF) and subsequently with ACM. DNA labeling revealed a marked stimulatory effect of ACM from 15 days in vitro (DIV) cultures in 30 DIV astrocytes after 12 h pre-treatment with growth factors. The main effects were found after INS or EGF pre-treatment in 30 DIV cultures. ACM collected from 15 or 60 or 90 DIV increased RNA labeling of 15 and 30 DIV astrocyte cultures, being the highest value that of 30 DIV cultures added with ACM from 90 DIV. The findings of increased DNA labeling after EGF or INS pre-treatment in 30 DIV cultures, followed by addition of ACM from 15 DIV cultures, suggest that these phenomena may depend by extra cellular signal-regulated kinase 1 (ERK1) activation.

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