Human Embryonic Stem Cells

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2006 Dec 5

PMID 17141030

Citations 5

Authors

Hidenori Akutsu

Chad A Cowan

Douglas Melton

Affiliations

Soon will be listed here.

Abstract

Human embryonic stem cells hold great promise in furthering our treatment of disease and increasing our understanding of early development. This chapter describes protocols for the derivation and maintenance of human embryonic stem cells. In addition, it summarizes briefly several alternative methods for the culture of human embryonic stem cells. Thus, this chapter provides a good starting point for researchers interested in harnessing the potential of human embryonic stem cells.

Citing Articles

Research and Development Strategy for Future Embryonic Stem Cell-Based Therapy in Japan.

Umezawa A, Sato Y, Kusakawa S, Amagase R, Akutsu H, Nakamura K JMA J. 2020; 3(4):287-294.

PMID: 33225099 PMC: 7676987. DOI: 10.31662/jmaj.2018-0029.

Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells.

Akutsu H, Machida M, Kanzaki S, Sugawara T, Ohkura T, Nakamura N Regen Ther. 2019; 1:18-29.

PMID: 31245438 PMC: 6581821. DOI: 10.1016/j.reth.2014.12.004.

Optimal timing of inner cell mass isolation increases the efficiency of human embryonic stem cell derivation and allows generation of sibling cell lines.

Chen A, Egli D, Niakan K, Deng J, Akutsu H, Yamaki M Cell Stem Cell. 2009; 4(2):103-6.

PMID: 19200798 PMC: 3335201. DOI: 10.1016/j.stem.2008.12.001.

Generation of human induced pluripotent stem cells from dermal fibroblasts.

Lowry W, Richter L, Yachechko R, Pyle A, Tchieu J, Sridharan R Proc Natl Acad Sci U S A. 2008; 105(8):2883-8.

PMID: 18287077 PMC: 2268554. DOI: 10.1073/pnas.0711983105.

Pedigreed primate embryonic stem cells express homogeneous familial gene profiles.

Navara C, Mich-Basso J, Redinger C, Ben-Yehudah A, Jacoby E, Kovkarova-Naumovski E Stem Cells. 2007; 25(11):2695-2704.

PMID: 17641389 PMC: 4357318. DOI: 10.1634/stemcells.2007-0286.