The Polypeptide Structure of Transmissible Gastroenteritis Virus

Overview

Journal J Gen Virol

Specialty Microbiology

Date 1975 Oct 1

PMID 171335

Citations 48

Authors

D J Garwes

D H Pocock

Affiliations

Soon will be listed here.

Abstract

The polypeptides of purified preparations of the coronavirus responsible for transmissible gastroenteritis of pigs have beem examined by polyacrylamide gel electrophoresis. Four major polypeptides, VPI (mol. wt. 200000), VP2 (50 000), VP3 (30000) and VP4 (28500) and two minor polypeptides, VPIa (105000) and VPIb (80500) have been reproducibly demonstrated in the virion, of which VPI, VP3 and VP4 contain carbohydrate. Treatment of the virion with the proteolytic enzyme bromelain removes the surface projections and VPI, thus identifying this glycopolypeptide as the major structural component of the projection.

Citing Articles

Porcine Respiratory Coronavirus as a Model for Acute Respiratory Coronavirus Disease.

Keep S, Carr B, Lean F, Fones A, Newman J, Dowgier G Front Immunol. 2022; 13:867707.

PMID: 35418984 PMC: 8995773. DOI: 10.3389/fimmu.2022.867707.

Minimum Determinants of Transmissible Gastroenteritis Virus Enteric Tropism Are Located in the N-Terminus of Spike Protein.

Sanchez C, Pascual-Iglesias A, Sola I, Zuniga S, Enjuanes L Pathogens. 2019; 9(1).

PMID: 31861369 PMC: 7168613. DOI: 10.3390/pathogens9010002.

Porcine deltacoronavirus enters cells via two pathways: A protease-mediated one at the cell surface and another facilitated by cathepsins in the endosome.

Zhang J, Chen J, Shi D, Shi H, Zhang X, Liu J J Biol Chem. 2019; 294(25):9830-9843.

PMID: 31068417 PMC: 6597833. DOI: 10.1074/jbc.RA119.007779.

Sequence of the nucleoprotein gene from a virulent British field isolate of transmissible gastroenteritis virus and its expression in Saccharomyces cerevisiae.

Britton P, Carmenes R, Page K, Garwes D, Parral F Mol Microbiol. 2017; 2(1):89-99.

PMID: 28776791 PMC: 7168467. DOI: 10.1111/j.1365-2958.1988.tb00010.x.

Entry from the cell surface of severe acute respiratory syndrome coronavirus with cleaved S protein as revealed by pseudotype virus bearing cleaved S protein.

Watanabe R, Matsuyama S, Shirato K, Maejima M, Fukushi S, Morikawa S J Virol. 2008; 82(23):11985-91.

PMID: 18786990 PMC: 2583654. DOI: 10.1128/JVI.01412-08.