Editing of Pre-mRNAs Can Occur Before Cis- and Trans-splicing in Petunia Mitochondria

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1991 Aug 1

PMID 1712907

Citations 28

Authors

C A Sutton

P L Conklin

K D Pruitt

M R Hanson

Affiliations

Soon will be listed here.

Abstract

Plant mitochondrial mRNAs have recently been shown to undergo editing, involving cytidine-to-uridine changes relative to the DNA sequence. We have examined the temporal relationship of editing and intron removal in coxII mRNAs in Petunia mitochondria. By using differential hybridization to probes specific for edited and unedited RNA and by sequencing of individual unspliced coxII pre-mRNA cDNAs, we found that RNA editing at any editing site can precede the splicing event. Similar results were obtained from examinations of pre-mRNA cDNAs of nad1, a gene composed of multiple exons that are both cis and trans spliced. Thus, intron removal is not required before editing can occur. The existence of editing intermediates indicates that the editing process is not strictly coincident with transcription.

Citing Articles

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ORRM5, an RNA recognition motif-containing protein, has a unique effect on mitochondrial RNA editing.

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Localized Retroprocessing as a Model of Intron Loss in the Plant Mitochondrial Genome.

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Increased accumulation of intron-containing transcripts in rice mitochondria caused by low temperature: is cold-sensitive RNA editing implicated?.

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Patterns of partial RNA editing in mitochondrial genes of Beta vulgaris.

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