M-current Noise and Putative M-channels in Cultured Rat Sympathetic Ganglion Cells

Overview

Journal J Physiol

Specialty Physiology

Date 1990 Dec 1

PMID 1712841

Citations 15

Authors

D G Owen

S J Marsh

D A Brown

Affiliations

Soon will be listed here.

Abstract

1. Whole-cell recordings of M-currents and single-channel recordings have been made in cultured rat sympathetic ganglion (SCG) neurones using the patch clamp technique. 2. Muscarine caused a reduction in macroscopic M-current relaxations, induced by voltage steps, and a concomitant reduction in whole-cell current noise. Power spectra of the muscarine-sensitive component of current noise were fitted with two Lorentzian components corresponding, on average, to 162 and 15 ms. The longer time constant was very similar to that of deactivation tail currents measured at the same potential. 3. The single-channel conductance at -30 mV was estimated from power density spectra and whole-cell current-variance relationships to be 1-2 pS. 4. Putative single M-channels, activated by depolarization, were identified in cell-attached and outside-out patches from cultured SCG neurones. In particular, the ensemble average of a small amplitude channel (estimated to be ca4 pS in physiological [K+]) in a cell-attached patch, exhibited a similar time dependence to whole-cell M-current.

Citing Articles

Kv7 Channels and Excitability Disorders.

Jones F, Gamper N, Gao H Handb Exp Pharmacol. 2021; 267:185-230.

PMID: 33860384 DOI: 10.1007/164_2021_457.

p75 and TrkA signaling regulates sympathetic neuronal firing patterns via differential modulation of voltage-gated currents.

Luther J, Birren S J Neurosci. 2009; 29(17):5411-24.

PMID: 19403809 PMC: 3326291. DOI: 10.1523/JNEUROSCI.3503-08.2009.

KCNQ/M currents in sensory neurons: significance for pain therapy.

Passmore G, Selyanko A, Mistry M, Al-Qatari M, Marsh S, Matthews E J Neurosci. 2003; 23(18):7227-36.

PMID: 12904483 PMC: 6740665.

Stoichiometry of expressed KCNQ2/KCNQ3 potassium channels and subunit composition of native ganglionic M channels deduced from block by tetraethylammonium.

Hadley J, Passmore G, Tatulian L, Al-Qatari M, Ye F, Wickenden A J Neurosci. 2003; 23(12):5012-9.

PMID: 12832524 PMC: 6741196.

Alternative splicing of KCNQ2 potassium channel transcripts contributes to the functional diversity of M-currents.

Pan Z, Selyanko A, Hadley J, Brown D, Dixon J, McKinnon D J Physiol. 2001; 531(Pt 2):347-58.

PMID: 11230508 PMC: 2278468. DOI: 10.1111/j.1469-7793.2001.0347i.x.

References

Hamill O, Marty A, Neher E, Sakmann B, Sigworth F . Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981; 391(2):85-100. DOI: 10.1007/BF00656997. View

Marrion N, Smart T, Brown D . Membrane currents in adult rat superior cervical ganglia in dissociated tissue culture. Neurosci Lett. 1987; 77(1):55-60. DOI: 10.1016/0304-3940(87)90606-9. View

Neher E, Stevens C . Conductance fluctuations and ionic pores in membranes. Annu Rev Biophys Bioeng. 1977; 6:345-81. DOI: 10.1146/annurev.bb.06.060177.002021. View

Pfaffinger P . Muscarine and t-LHRH suppress M-current by activating an IAP-insensitive G-protein. J Neurosci. 1988; 8(9):3343-53. PMC: 6569448. View

Lancaster B, Pennefather P . Potassium currents evoked by brief depolarizations in bull-frog sympathetic ganglion cells. J Physiol. 1987; 387:519-48. PMC: 1192518. DOI: 10.1113/jphysiol.1987.sp016587. View

Lopez H, Adams P . A G Protein Mediates the Inhibition of the Voltage-Dependent Potassium M Current by Muscarine, LHRH, Substance P and UTP in Bullfrog Sympathetic Neurons. Eur J Neurosci. 1989; 1(5):529-542. DOI: 10.1111/j.1460-9568.1989.tb00360.x. View

Brown D, Marrion N, Smart T . On the transduction mechanism for muscarine-induced inhibition of M-current in cultured rat sympathetic neurones. J Physiol. 1989; 413:469-88. PMC: 1189111. DOI: 10.1113/jphysiol.1989.sp017664. View

Constanti A, Brown D . M-Currents in voltage-clamped mammalian sympathetic neurones. Neurosci Lett. 1981; 24(3):289-94. DOI: 10.1016/0304-3940(81)90173-7. View

Sigworth F . The variance of sodium current fluctuations at the node of Ranvier. J Physiol. 1980; 307:97-129. PMC: 1283036. DOI: 10.1113/jphysiol.1980.sp013426. View

10.

Neher E, Marty A, Fukuda K, Kubo T, Numa S . Intracellular calcium release mediated by two muscarinic receptor subtypes. FEBS Lett. 1988; 240(1-2):88-94. DOI: 10.1016/0014-5793(88)80345-4. View

11.

Brown D, Selyanko A . Two components of muscarine-sensitive membrane current in rat sympathetic neurones. J Physiol. 1985; 358:335-63. PMC: 1193345. DOI: 10.1113/jphysiol.1985.sp015554. View

12.

Capiod T, Ogden D . The properties of calcium-activated potassium ion channels in guinea-pig isolated hepatocytes. J Physiol. 1989; 409:285-95. PMC: 1190444. DOI: 10.1113/jphysiol.1989.sp017497. View

13.

Brown D, Adams P . Muscarinic suppression of a novel voltage-sensitive K+ current in a vertebrate neurone. Nature. 1980; 283(5748):673-6. DOI: 10.1038/283673a0. View

14.

Belardetti F, Kandel E, Siegelbaum S . Neuronal inhibition by the peptide FMRFamide involves opening of S K+ channels. Nature. 1987; 325(7000):153-6. DOI: 10.1038/325153a0. View

15.

Adams P, Brown D, Constanti A . M-currents and other potassium currents in bullfrog sympathetic neurones. J Physiol. 1982; 330:537-72. PMC: 1225314. DOI: 10.1113/jphysiol.1982.sp014357. View

16.

Colquhoun D, Dreyer F, Sheridan R . The actions of tubocurarine at the frog neuromuscular junction. J Physiol. 1979; 293:247-84. PMC: 1280712. DOI: 10.1113/jphysiol.1979.sp012888. View

17.

Adams P, Brown D, Constanti A . Pharmacological inhibition of the M-current. J Physiol. 1982; 332:223-62. PMC: 1197396. DOI: 10.1113/jphysiol.1982.sp014411. View

18.

Marty A, Tan Y, Trautmann A . Three types of calcium-dependent channel in rat lacrimal glands. J Physiol. 1984; 357:293-325. PMC: 1193259. DOI: 10.1113/jphysiol.1984.sp015501. View

19.

HODGKIN A, Katz B . The effect of sodium ions on the electrical activity of giant axon of the squid. J Physiol. 1949; 108(1):37-77. PMC: 1392331. DOI: 10.1113/jphysiol.1949.sp004310. View