Human Papillomavirus (HPV) Types 101 and 103 Isolated from Cervicovaginal Cells Lack an E6 Open Reading Frame (ORF) and Are Related to Gamma-papillomaviruses

Overview

Journal Virology

Specialty Microbiology

Date 2006 Nov 28

PMID 17125811

Citations 34

Authors

Zigui Chen

Mark Schiffman

Rolando Herrero

Rob DeSalle

Robert D Burk

Affiliations

Soon will be listed here.

Abstract

Complete genomes of HPV101 and HPV103 were PCR amplified and cloned from cervicovaginal cells of a 34-year-old female with cervical intraepithelial neoplasia grade 3 (CIN 3) and a 30-year-old female with a normal Pap test, respectively. HPV101 and HPV103 contain 4 early genes (E7, E1, E2, and E4) and 2 late genes (L2 and L1), but both lack the canonical E6 ORF. Pairwise alignment similarity of the L1 ORF nucleotide sequences of HPV101 and HPV103 indicated that they are at least 30% dissimilar to each other and all known PVs. However, similarities of the other ORFs (E7, E1, E2, and L2) indicated that HPV101 and HPV103 are most related to each other. Phylogenetic analyses revealed that these two types form a monophyletic clade, clustering together with the gamma- and pi-PV groups. These data demonstrated that HPV genomes closely related to papillomaviruses identified from cutaneous epithelia can be isolated from the genital mucosal region. Moreover, this is the first report of HPVs lacking an E6 ORF and phylogenetic evidence suggests this occurred subsequent to their emergence from the gamma-/pi-PVs.

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References

Chan W, Chong T, Bernard H, Klock G . Transcription of the transforming genes of the oncogenic human papillomavirus-16 is stimulated by tumor promotors through AP1 binding sites. Nucleic Acids Res. 1990; 18(4):763-9. PMC: 330325. DOI: 10.1093/nar/18.4.763. View

Dong X, Stubenrauch F, Pfister H . Prevalence of deletions of YY1-binding sites in episomal HPV 16 DNA from cervical cancers. Int J Cancer. 1994; 58(6):803-8. DOI: 10.1002/ijc.2910580609. View

Jackson M, Pennie W, McCaffery R, Smith K, Grindlay G, Campo M . The B subgroup bovine papillomaviruses lack an identifiable E6 open reading frame. Mol Carcinog. 1991; 4(5):382-7. DOI: 10.1002/mc.2940040510. View

Ishiji T, Lace M, Parkkinen S, Anderson R, Haugen T, Cripe T . Transcriptional enhancer factor (TEF)-1 and its cell-specific co-activator activate human papillomavirus-16 E6 and E7 oncogene transcription in keratinocytes and cervical carcinoma cells. EMBO J. 1992; 11(6):2271-81. PMC: 556694. DOI: 10.1002/j.1460-2075.1992.tb05286.x. View

Andrews P . Evolution and environment in the Hominoidea. Nature. 1992; 360(6405):641-6. DOI: 10.1038/360641a0. View

Chan S, Delius H, Halpern A, Bernard H . Analysis of genomic sequences of 95 papillomavirus types: uniting typing, phylogeny, and taxonomy. J Virol. 1995; 69(5):3074-83. PMC: 189008. DOI: 10.1128/JVI.69.5.3074-3083.1995. View

Thompson J, Gibson T, Plewniak F, Jeanmougin F, Higgins D . The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1998; 25(24):4876-82. PMC: 147148. DOI: 10.1093/nar/25.24.4876. View

Delius H, Saegling B, Bergmann K, Shamanin V, de Villiers E . The genomes of three of four novel HPV types, defined by differences of their L1 genes, show high conservation of the E7 gene and the URR. Virology. 1998; 240(2):359-65. DOI: 10.1006/viro.1997.8943. View

Forslund O, Antonsson A, Nordin P, Stenquist B, Hansson B . A broad range of human papillomavirus types detected with a general PCR method suitable for analysis of cutaneous tumours and normal skin. J Gen Virol. 1999; 80 ( Pt 9):2437-2443. DOI: 10.1099/0022-1317-80-9-2437. View

10.

Narechania A, Chen Z, DeSalle R, Burk R . Phylogenetic incongruence among oncogenic genital alpha human papillomaviruses. J Virol. 2005; 79(24):15503-10. PMC: 1316001. DOI: 10.1128/JVI.79.24.15503-15510.2005. View

11.

Herrero R, Hildesheim A, Bratti C, Sherman M, Hutchinson M, Morales J . Population-based study of human papillomavirus infection and cervical neoplasia in rural Costa Rica. J Natl Cancer Inst. 2000; 92(6):464-74. DOI: 10.1093/jnci/92.6.464. View

12.

Castresana J . Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000; 17(4):540-52. DOI: 10.1093/oxfordjournals.molbev.a026334. View

13.

Terai M, Burk R . Complete nucleotide sequence and analysis of a novel human papillomavirus (HPV 84) genome cloned by an overlapping PCR method. Virology. 2001; 279(1):109-15. DOI: 10.1006/viro.2000.0716. View

14.

Terai M, Burk R . Characterization of a novel genital human papillomavirus by overlapping PCR: candHPV86 identified in cervicovaginal cells of a woman with cervical neoplasia. J Gen Virol. 2001; 82(Pt 9):2035-2040. DOI: 10.1099/0022-1317-82-9-2035. View

15.

Huelsenbeck J, Ronquist F . MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001; 17(8):754-5. DOI: 10.1093/bioinformatics/17.8.754. View

16.

Terai M, DeSalle R, Burk R . Lack of canonical E6 and E7 open reading frames in bird papillomaviruses: Fringilla coelebs papillomavirus and Psittacus erithacus timneh papillomavirus. J Virol. 2002; 76(19):10020-3. PMC: 136527. DOI: 10.1128/jvi.76.19.10020-10023.2002. View

17.

Antonsson A, Hansson B . Healthy skin of many animal species harbors papillomaviruses which are closely related to their human counterparts. J Virol. 2002; 76(24):12537-42. PMC: 136724. DOI: 10.1128/jvi.76.24.12537-12542.2002. View

18.

Clifford G, Smith J, Plummer M, Munoz N, Franceschi S . Human papillomavirus types in invasive cervical cancer worldwide: a meta-analysis. Br J Cancer. 2003; 88(1):63-73. PMC: 2376782. DOI: 10.1038/sj.bjc.6600688. View

19.

Munoz N, Bosch F, de Sanjose S, Herrero R, Castellsague X, Shah K . Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med. 2003; 348(6):518-27. DOI: 10.1056/NEJMoa021641. View

20.

Antonsson A, Erfurt C, Hazard K, Holmgren V, Simon M, Kataoka A . Prevalence and type spectrum of human papillomaviruses in healthy skin samples collected in three continents. J Gen Virol. 2003; 84(Pt 7):1881-1886. DOI: 10.1099/vir.0.18836-0. View