CD80/CD28 Co-stimulation in Human Brucellosis

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 2006 Nov 15

PMID 17100758

Citations 5

Authors

P Skendros

P Boura

F Kamaria

M Raptopoulou-Gigi

Affiliations

Soon will be listed here.

Abstract

Despite treatment, 10-30% of brucellosis patients develop chronic disease, characterized by atypical clinical picture and/or relapses. A defective T helper 1 (Th1) response and a low [corrected] percentage of CD4(+)/CD25(+) cells have been described in chronic brucellosis patients. CD80/CD28 co-stimulation is critical for an efficient Th1 response and has not been studied previously in human brucellosis. In order to investigate the role of CD80/CD28 co-stimulation, 13 acute brucellosis patients (AB), 22 chronic brucellosis patients (CB, 12/22 relapsing type-CB1 and 10/22 atypical type-CB2), 11 'cured' subjects and 15 healthy volunteers (controls) were studied. The percentage of CD4(+)/CD28(+) T lymphocytes and CD14(+)/CD80(+) monocytes were analysed by flow cytometry both ex vivo and after phytohaemagglutinin (PHA)-stimulation with or without heat-killed Brucella abortus (HkBA). Ex vivo analysis showed no differences between all groups studied. PHA stimulation up-regulated the percentage of CD80(+) monocytes in AB compared to 'cured' subjects and controls (P < 0.001), although the proportion of CD4(+)/CD28(+) cells did not alter. A higher percentage of CD80(+) monocytes was observed in the CB1 subgroup, compared to AB, 'cured' subjects and controls (P = 0.042, < 0.001 and < 0.001, respectively). CB2 was characterized by a lower percentage of CD80(+) monocytes in comparison to CB1 (P = 0.020). HkBA in PHA cultures down-regulated the percentage of CD80(+) monocytes compared to PHA alone in all groups, especially in AB and CB patients (P < 0.001 and P = 0.007, respectively). In conclusion, the diminished percentage of CD4(+)/CD25(+) T cells in CB is not associated with inadequate CD80/CD28 co-stimulation. We speculate that differential frequency of CD80(+) monocytes after PHA stimulation could serve as a qualitative parameter of disease status, related to the different clinical forms of chronic brucellosis.

Citing Articles

Dysregulates Monocytes and Inhibits Macrophage Polarization through LC3-Dependent Autophagy.

Wang Y, Li Y, Li H, Song H, Zhai N, Lou L Front Immunol. 2017; 8:691.

PMID: 28659924 PMC: 5467008. DOI: 10.3389/fimmu.2017.00691.

Evaluation of Regulatory T Cells in Patients with Acute and Chronic Brucellosis.

Ganji A, Mosayebi G, Ghaznavi-Rad E, Khosravi K, Zarinfar N Rep Biochem Mol Biol. 2017; 5(2):91-96.

PMID: 28367469 PMC: 5346275.

Frequencies of CD4+ T Regulatory Cells and their CD25(high) and FoxP3(high) Subsets Augment in Peripheral Blood of Patients with Acute and Chronic Brucellosis.

Bahador A, Hadjati J, Hassannejad N, Ghazanfari H, Maracy M, Jafari S Osong Public Health Res Perspect. 2014; 5(3):161-8.

PMID: 25180149 PMC: 4147229. DOI: 10.1016/j.phrp.2014.04.008.

Discordant Brucella melitensis antigens yield cognate CD8+ T cells in vivo.

Durward M, Harms J, Magnani D, Eskra L, Splitter G Infect Immun. 2009; 78(1):168-76.

PMID: 19884330 PMC: 2798188. DOI: 10.1128/IAI.00994-09.

Chronic brucellosis patients retain low frequency of CD4+ T-lymphocytes expressing CD25 and CD28 after Escherichia coli LPS stimulation of PHA-cultured PBMCs.

Skendros P, Sarantopoulos A, Tselios K, Boura P Clin Dev Immunol. 2009; 2008:327346.

PMID: 19190764 PMC: 2630417. DOI: 10.1155/2008/327346.

References

Corominas M, Cardona V, Gonzalez L, Cayla J, Rufi G, Mestre M . B-lymphocytes and co-stimulatory molecules in Mycobacterium tuberculosis infection. Int J Tuberc Lung Dis. 2004; 8(1):98-105. View

Giambartolomei G, Delpino M, Cahanovich M, Wallach J, Baldi P, Velikovsky C . Diminished production of T helper 1 cytokines correlates with T cell unresponsiveness to Brucella cytoplasmic proteins in chronic human brucellosis. J Infect Dis. 2002; 186(2):252-9. DOI: 10.1086/341449. View

Pappas G, Akritidis N, Bosilkovski M, Tsianos E . Brucellosis. N Engl J Med. 2005; 352(22):2325-36. DOI: 10.1056/NEJMra050570. View

Dobrovolskaia M, Vogel S . Toll receptors, CD14, and macrophage activation and deactivation by LPS. Microbes Infect. 2002; 4(9):903-14. DOI: 10.1016/s1286-4579(02)01613-1. View

Acuto O, Michel F . CD28-mediated co-stimulation: a quantitative support for TCR signalling. Nat Rev Immunol. 2003; 3(12):939-51. DOI: 10.1038/nri1248. View

Thornes R, Early A, Hogan B, Reen D . Chronic brucellosis--clinical response to reduction of suppressor T lymphocytes by cyclophosphamide/prednisone. Ir Med J. 1982; 75(11):423-4. View

Fernandez-Prada C, Nikolich M, Vemulapalli R, Sriranganathan N, Boyle S, Schurig G . Deletion of wboA enhances activation of the lectin pathway of complement in Brucella abortus and Brucella melitensis. Infect Immun. 2001; 69(7):4407-16. PMC: 98513. DOI: 10.1128/IAI.69.7.4407-4416.2001. View

Agranovich I, Scott D, Terle D, Lee K, Golding B . Down-regulation of Th2 responses by Brucella abortus, a strong Th1 stimulus, correlates with alterations in the B7.2-CD28 pathway. Infect Immun. 1999; 67(9):4418-26. PMC: 96760. DOI: 10.1128/IAI.67.9.4418-4426.1999. View

Pentcheva-Hoang T, Egen J, Wojnoonski K, Allison J . B7-1 and B7-2 selectively recruit CTLA-4 and CD28 to the immunological synapse. Immunity. 2004; 21(3):401-13. DOI: 10.1016/j.immuni.2004.06.017. View

10.

Inoue S, Golding B, Scott D . Programming of CTL with heat-killed Brucella abortus and antigen allows soluble antigen alone to generate effective secondary CTL. Vaccine. 2005; 23(14):1730-8. DOI: 10.1016/j.vaccine.2004.09.034. View

11.

Agrewala J, Kumar B, Vohra H . Potential role of B7-1 and CD28 molecules in immunosuppression in leprosy. Clin Exp Immunol. 1998; 111(1):56-63. PMC: 1904849. DOI: 10.1046/j.1365-2249.1998.00463.x. View

12.

Reiser H, Stadecker M . Costimulatory B7 molecules in the pathogenesis of infectious and autoimmune diseases. N Engl J Med. 1996; 335(18):1369-77. DOI: 10.1056/NEJM199610313351807. View

13.

Kariminia A, Kavoossy G, Khatami S, Zowghi E, Ardestani S . Study of interleukin-10 and interleukin-12 productions in response to lipopolysaccharides extracted from two different Brucella strains. Comp Immunol Microbiol Infect Dis. 2002; 25(2):85-93. DOI: 10.1016/s0147-9571(01)00029-7. View

14.

Skendros P, Boura P, Chrisagis D, Raptopoulou-Gigi M . Diminished percentage of CD4+ T-lymphocytes expressing interleukine-2 receptor alpha in chronic brucellosis. J Infect. 2006; 54(2):192-7. DOI: 10.1016/j.jinf.2006.04.001. View

15.

Bossi P, Tegnell A, Baka A, Loock F, Hendriks J, Werner A . Bichat guidelines for the clinical management of brucellosis and bioterrorism-related brucellosis. Euro Surveill. 2005; 9(12):E15-6. View

16.

Dornand J, Gross A, Lafont V, Liautard J, Oliaro J, Liautard J . The innate immune response against Brucella in humans. Vet Microbiol. 2002; 90(1-4):383-94. DOI: 10.1016/s0378-1135(02)00223-7. View

17.

Bhatia S, Edidin M, Almo S, Nathenson S . B7-1 and B7-2: similar costimulatory ligands with different biochemical, oligomeric and signaling properties. Immunol Lett. 2006; 104(1-2):70-5. DOI: 10.1016/j.imlet.2005.11.019. View

18.

Rafiei A, Ardestani S, Kariminia A, Keyhani A, Mohraz M, Amirkhani A . Dominant Th1 cytokine production in early onset of human brucellosis followed by switching towards Th2 along prolongation of disease. J Infect. 2006; 53(5):315-24. DOI: 10.1016/j.jinf.2005.11.024. View

19.

Oliveira S, Splitter G . CD8+ type 1 CD44hi CD45 RBlo T lymphocytes control intracellular Brucella abortus infection as demonstrated in major histocompatibility complex class I- and class II-deficient mice. Eur J Immunol. 1995; 25(9):2551-7. DOI: 10.1002/eji.1830250922. View

20.

Kinikli S, Turkcapar N, Kucukay M, Keskin G, Kinikli G . In vitro nonspecific mitogenic response of T-cell subsets in acute and chronic brucellosis. Diagn Microbiol Infect Dis. 2005; 52(3):229-33. DOI: 10.1016/j.diagmicrobio.2005.02.016. View