An Immunocompetent Mouse Model for the Tolerance of Human Chronic Hepatitis B Virus Infection

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 Nov 11

PMID 17095599

Citations 144

Authors

Li-Rung Huang

Hui-Lin Wu

Pei-Jer Chen

Ding-Shinn Chen

Affiliations

Soon will be listed here.

Abstract

An animal model for human hepatitis B virus (HBV) tolerance is needed to investigate the mechanisms. This model will also facilitate therapeutic strategies for the existing 350 million patients with chronic hepatitis B. We established a mouse model by hydrodynamic injection of an engineered, replication-competent HBV DNA into the tail veins of C57BL/6 mice. In 40% of the injected mice, HBV surface antigenemia persisted for > 6 months. Viral replication intermediates, transcripts, and proteins were detected in the liver tissues of the injected mice for up to 1 year. The tolerance toward HBV surface antigen in this model was shown to be due to an insufficient cellular immunity against hepatitis B core antigen, as was documented in humans. This animal model will accelerate further genetic and mechanistic studies of human chronic hepatitis B infection.

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