Accuracy of Enzyme-linked Immunosorbent Assay Using Crude and Purified Antigens for Serodiagnosis of Melioidosis

Overview

Journal Clin Vaccine Immunol

Publisher American Society for Microbiology

Specialties Allergy & Immunology
Pathology

Date 2006 Nov 10

PMID 17093104

Citations 26

Authors

Narisara Chantratita

Vanaporn Wuthiekanun

Aunchalee Thanwisai

Direk Limmathurotsakul

Allen C Cheng

Wirongrong Chierakul

Nicholas P J Day

Sharon J Peacock

Affiliations

Soon will be listed here.

Abstract

Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).

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