Inhibitory Effect of Chitooligosaccharides on Matrix Metalloproteinase-9 in Human Fibrosarcoma Cells (HT1080)

Overview

Journal Mar Biotechnol (NY)

Specialties Biology
Biotechnology

Date 2006 Nov 9

PMID 17091328

Citations 8

Authors

Quang Van Ta

Moon-Moo Kim

Se-Kwon Kim

Affiliations

Soon will be listed here.

Abstract

Matrix metalloproteinase-9 (MMP-9) has gelatinase activity and plays an important role in cancer invasion and metastasis. Therefore, inhibition of specific types of MMPs including MMP-9 has become an attractive target for therapeutic intervention. The aim of this study was to investigate the effect of chitooligosaccharides (COS) on activity and expression of MMP-9 in HT1080 cells. The inhibitory effect of COS with different molecular masses was examined by gelatin zymography, reverse transcriptase-polymerase chain reaction (RT-PCR), gene reporter assay, and Western blot analysis. MMP-9 inhibition in the presence of COS was clearly observed in gelatin zymography. Specifically, 1- to 3-kDa COS (COS-I) exhibited the highest inhibitory effect on MMP-9 activity in HT1080 cells among tested molecular mass fractions. It was also found that COS-I was capable of inhibiting both gene and protein expression of MMP-9 (P<0.01). These results suggest that low molecular mass COS can be considered as a potent inhibitor of MMP-9.

Citing Articles

Antimicrobial and anticarcinogenic activity of bioactive peptides derived from abalone viscera (Haliotis fulgens and Haliotis corrugata).

Rivera-Perez C, Ponce Gonzalez X, Hernandez-Savedra N Sci Rep. 2023; 13(1):15185.

PMID: 37704667 PMC: 10499822. DOI: 10.1038/s41598-023-41491-w.

Cloning and Characterization of a New Chitosanase From a Deep-Sea Bacterium sp. QD07.

Zheng Q, Meng X, Cheng M, Li Y, Liu Y, Chen X Front Microbiol. 2021; 12:619731.

PMID: 33717008 PMC: 7943732. DOI: 10.3389/fmicb.2021.619731.

Downregulation of CD147 by chitooligosaccharide inhibits MMP-2 expression and suppresses the metastatic potential of human gastric cancer.

Luo Z, Dong X, Ke Q, Duan Q, Shen L Oncol Lett. 2014; 8(1):361-366.

PMID: 24959277 PMC: 4063597. DOI: 10.3892/ol.2014.2115.

Biocompatibility and Alkaline Phosphatase Activity of Phosphorylated Chitooligosaccharides on the Osteosarcoma MG63 Cell Line.

Venkatesan J, Pangestuti R, Qian Z, Ryu B, Kim S J Funct Biomater. 2014; 1(1):3-13.

PMID: 24955930 PMC: 4030895. DOI: 10.3390/jfb1010003.

Marine antitumor drugs: status, shortfalls and strategies.

Bhatnagar I, Kim S Mar Drugs. 2010; 8(10):2702-20.

PMID: 21116415 PMC: 2993001. DOI: 10.3390/md8102702.

References

Benbow U, Brinckerhoff C . The AP-1 site and MMP gene regulation: what is all the fuss about?. Matrix Biol. 1997; 15(8-9):519-26. DOI: 10.1016/s0945-053x(97)90026-3. View

Lee S, Suh J, Kim H, Lee J, Song J, Lee S . MR evaluation of radiation synovectomy of the knee by means of intra-articular injection of holmium-166-chitosan complex in patients with rheumatoid arthritis: results at 4-month follow-up. Korean J Radiol. 2003; 4(3):170-8. PMC: 2698084. DOI: 10.3348/kjr.2003.4.3.170. View

Bjorklund M, Koivunen E . Gelatinase-mediated migration and invasion of cancer cells. Biochim Biophys Acta. 2005; 1755(1):37-69. DOI: 10.1016/j.bbcan.2005.03.001. View

Botos I, Scapozza L, Zhang D, Liotta L, Meyer E . Batimastat, a potent matrix mealloproteinase inhibitor, exhibits an unexpected mode of binding. Proc Natl Acad Sci U S A. 1996; 93(7):2749-54. PMC: 39703. DOI: 10.1073/pnas.93.7.2749. View

Curran S, Murray G . Matrix metalloproteinases in tumour invasion and metastasis. J Pathol. 1999; 189(3):300-8. DOI: 10.1002/(SICI)1096-9896(199911)189:3<300::AID-PATH456>3.0.CO;2-C. View

Hansen M, Nielsen S, Berg K . Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods. 1989; 119(2):203-10. DOI: 10.1016/0022-1759(89)90397-9. View

Nelson A, Fingleton B, Rothenberg M, Matrisian L . Matrix metalloproteinases: biologic activity and clinical implications. J Clin Oncol. 2000; 18(5):1135-49. DOI: 10.1200/JCO.2000.18.5.1135. View

Qin C, Du Y, Xiao L, Li Z, Gao X . Enzymic preparation of water-soluble chitosan and their antitumor activity. Int J Biol Macromol. 2003; 31(1-3):111-7. DOI: 10.1016/s0141-8130(02)00064-8. View

Ala-Aho R, Kahari V . Collagenases in cancer. Biochimie. 2005; 87(3-4):273-86. DOI: 10.1016/j.biochi.2004.12.009. View

10.

Liao J, Wolfman J, Wolfman A . K-ras regulates the steady-state expression of matrix metalloproteinase 2 in fibroblasts. J Biol Chem. 2003; 278(34):31871-8. DOI: 10.1074/jbc.M301931200. View

11.

Itoh T, Tanioka M, Yoshida H, Yoshioka T, Nishimoto H, Itohara S . Reduced angiogenesis and tumor progression in gelatinase A-deficient mice. Cancer Res. 1998; 58(5):1048-51. View

12.

Cuniasse P, Devel L, Makaritis A, Beau F, Georgiadis D, Matziari M . Future challenges facing the development of specific active-site-directed synthetic inhibitors of MMPs. Biochimie. 2005; 87(3-4):393-402. DOI: 10.1016/j.biochi.2004.09.025. View

13.

Fabunmi R, Baker A, Murray E, Booth R, Newby A . Divergent regulation by growth factors and cytokines of 95 kDa and 72 kDa gelatinases and tissue inhibitors or metalloproteinases-1, -2, and -3 in rabbit aortic smooth muscle cells. Biochem J. 1996; 315 ( Pt 1):335-42. PMC: 1217192. DOI: 10.1042/bj3150335. View

14.

Park P, Je J, Kim S . Angiotensin I converting enzyme (ACE) inhibitory activity of hetero-chitooligosaccharides prepared from partially different deacetylated chitosans. J Agric Food Chem. 2003; 51(17):4930-4. DOI: 10.1021/jf0340557. View

15.

Klein G, Vellenga E, Fraaije M, Kamps W, de Bont E . The possible role of matrix metalloproteinase (MMP)-2 and MMP-9 in cancer, e.g. acute leukemia. Crit Rev Oncol Hematol. 2004; 50(2):87-100. DOI: 10.1016/j.critrevonc.2003.09.001. View

16.

Mook O, Frederiks W, van Noorden C . The role of gelatinases in colorectal cancer progression and metastasis. Biochim Biophys Acta. 2004; 1705(2):69-89. DOI: 10.1016/j.bbcan.2004.09.006. View

17.

Shinozaki Y, Akutsu-Shigeno Y, Nakajima-Kambe T, Inomata S, Nomura N, Nakahara T . Inhibition of matrix metalloproteinase-2 activity by siderophores of Pseudomonas species. Appl Microbiol Biotechnol. 2004; 64(6):840-7. DOI: 10.1007/s00253-003-1521-2. View

18.

Martel-Pelletier J, Welsch D, Pelletier J . Metalloproteases and inhibitors in arthritic diseases. Best Pract Res Clin Rheumatol. 2002; 15(5):805-29. DOI: 10.1053/berh.2001.0195. View

19.

Hrabec E, Strek M, Nowak D, Greger J, Suwalski M, Hrabec Z . Activity of type IV collagenases (MMP-2 and MMP-9) in primary pulmonary carcinomas: a quantitative analysis. J Cancer Res Clin Oncol. 2002; 128(4):197-204. DOI: 10.1007/s00432-001-0320-3. View

20.

Zhang L, Shi J, Feng J, Klocker H, Lee C, Zhang J . Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer. Prostate Cancer Prostatic Dis. 2004; 7(4):327-32. DOI: 10.1038/sj.pcan.4500750. View