Nonhematopoietic Erythropoietin Derivatives Prevent Motoneuron Degeneration in Vitro and in Vivo

Overview

Journal Mol Med

Publisher Biomed Central

Specialties General Medicine
Molecular Biology

Date 2006 Nov 8

PMID 17088947

Citations 42

Authors

Tiziana Mennini

Massimiliano De Paola

Paolo Bigini

Cristina Mastrotto

Elena Fumagalli

Sara Barbera

Manuela Mengozzi

Barbara Viviani

Emanuela Corsini

Marina Marinovich

Lars Torup

Johan van Beek

Marcel Leist

Michael Brines

Antony Cerami

Pietro Ghezzi

Affiliations

Soon will be listed here.

Abstract

Chronic treatment with asialo erythropoietin (ASIALO-EPO) or carbamylated erythropoietin (CEPO) improved motor behavior and reduced motoneuron loss and astrocyte and microglia activation in the cervical spinal cord of wobbler mice, an animal model of amyotrophic lateral sclerosis, but had no effect on hematocrit values. ASIALO-EPO and CEPO, like the parent compound EPO, protected primary motoneuron cultures from kainate-induced death in vitro. Both EPO receptor and the common CD131 beta chain were expressed in cultured motoneurons and in the anterior horn of wobbler mice spinal cord. Our results strongly support a role for the common beta chain CD131 in the protective effect of EPO derivatives on motoneuron degeneration. Thus CEPO, which does not bind to the classical homodimeric EPO receptor and is devoid of hematopoietic activity, could be effective in chronic treatment aimed at reducing motoneuron degeneration.

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