Crimean-Congo Hemorrhagic Fever Virus: Genetic Analysis and Tick Survey in Turkey

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2006 Nov 8

PMID 17088370

Citations 34

Authors

Sukru Tonbak

Munir Aktas

Kursat Altay

Ahmet K Azkur

Ahmet Kalkan

Yusuf Bolat

Nazir Dumanli

Aykut Ozdarendeli

Affiliations

Soon will be listed here.

Abstract

Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus in the family Bunyaviridae, genus Nairovirus. The virus is transmitted to humans through infected tick bites or from direct contact with viremic animals or humans. In the present study, a total of 1,015 adult ticks were collected from cattle (603 specimens), sheep (17 specimens), and goats (395 specimens) in the Kelkit Valley in Turkey. Four tick species were recognized on the animals in the surveyed region. The most abundant species were Rhipicephalus bursa and Hyalomma marginatum marginatum, at 47.68% (484/1,015) and 46.40% (471/1,015), respectively. Reverse transcriptase PCR was used to recover partial sequences of the CCHFV small (S) genome segment. The presence of CCHFV was determined in 3 of 33 (9.09%) R. bursa pools and in 1 of 31 (3.22%) H. m. marginatum pools. Virus sequences from R. bursa were extremely different from those of the Greek CCHFV strain (U04958) isolated from an R. bursa tick. Phylogenetic analysis indicated that the CCHFV isolates obtained in this study clustered in group 5, whose range encompasses southwestern Russian and Kosovo. This is the first evidence of CCHFV in ticks from Turkey. Even though Hyalomma is the main vector for CCHFV, R. bursa may play a role in CCHFV transmission.

Citing Articles

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References

Drosten C, Minnak D, Emmerich P, Schmitz H, Reinicke T . Crimean-Congo hemorrhagic fever in Kosovo. J Clin Microbiol. 2002; 40(3):1122-3. PMC: 120293. DOI: 10.1128/JCM.40.3.1122-1123.2002. View

Aktas M, Altay K, Dumanli N . A molecular survey of bovine Theileria parasites among apparently healthy cattle and with a note on the distribution of ticks in eastern Turkey. Vet Parasitol. 2006; 138(3-4):179-85. DOI: 10.1016/j.vetpar.2006.01.052. View

Papa A, Bino S, Llagami A, Brahimaj B, Papadimitriou E, Pavlidou V . Crimean-Congo hemorrhagic fever in Albania, 2001. Eur J Clin Microbiol Infect Dis. 2002; 21(8):603-6. DOI: 10.1007/s10096-002-0770-9. View

Yashina L, Vyshemirskii O, Seregin S, Petrova I, Samokhvalov E, Lvov D . Genetic analysis of Crimean-Congo hemorrhagic fever virus in Russia. J Clin Microbiol. 2003; 41(2):860-2. PMC: 149693. DOI: 10.1128/JCM.41.2.860-862.2003. View

Yashina L, Petrova I, Seregin S, Vyshemirskii O, Lvov D, Aristova V . Genetic variability of Crimean-Congo haemorrhagic fever virus in Russia and Central Asia. J Gen Virol. 2003; 84(Pt 5):1199-1206. DOI: 10.1099/vir.0.18805-0. View

Grigorev M, Evchenko I, Shaposhnikova L, Shchenetts K, Emelianov S, Ermolova N . [Role of some wild birds and mammals in the natural foci of Crimean haemorrhagic fever in Stavropol' region]. Zh Mikrobiol Epidemiol Immunobiol. 2003; (6 Suppl):92-5. View

Kotti B, Shaposhnikova L, Evchenko I, Levchenko B, Surkhaev D, Korzhov P . [Hyalomma marginatum Koch in Stavropol' region]. Zh Mikrobiol Epidemiol Immunobiol. 2003; (6 Suppl):105-8. View

Aktas M, Dumanli N, Angin M . Cattle infestation by Hyalomma ticks and prevalence of Theileria in Hyalomma species in the east of Turkey. Vet Parasitol. 2004; 119(1):1-8. DOI: 10.1016/j.vetpar.2003.10.013. View

Hewson R, Chamberlain J, Mioulet V, Lloyd G, Jamil B, Hasan R . Crimean-Congo haemorrhagic fever virus: sequence analysis of the small RNA segments from a collection of viruses world wide. Virus Res. 2004; 102(2):185-9. DOI: 10.1016/j.virusres.2003.12.035. View

10.

Chinikar S, Persson S, Johansson M, Bladh L, Goya M, Houshmand B . Genetic analysis of Crimean-congo hemorrhagic fever virus in Iran. J Med Virol. 2004; 73(3):404-11. DOI: 10.1002/jmv.20106. View

11.

Gozalan A, Akin L, Rolain J, Tapar F, Oncul O, Yoshikura H . [Epidemiological evaluation of a possible outbreak in and nearby Tokat province]. Mikrobiyol Bul. 2004; 38(1-2):33-44. View

12.

Karti S, Odabasi Z, Korten V, Yilmaz M, Sonmez M, Caylan R . Crimean-Congo hemorrhagic fever in Turkey. Emerg Infect Dis. 2004; 10(8):1379-84. PMC: 3320426. DOI: 10.3201/eid1008.030928. View

13.

Burney M, Ghafoor A, Saleen M, WEBB P, Casals J . Nosocomial outbreak of viral hemorrhagic fever caused by Crimean Hemorrhagic fever-Congo virus in Pakistan, January 1976. Am J Trop Med Hyg. 1980; 29(5):941-7. DOI: 10.4269/ajtmh.1980.29.941. View

14.

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

15.

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View

16.

Rodriguez L, Maupin G, Ksiazek T, Rollin P, Khan A, Schwarz T . Molecular investigation of a multisource outbreak of Crimean-Congo hemorrhagic fever in the United Arab Emirates. Am J Trop Med Hyg. 1997; 57(5):512-8. DOI: 10.4269/ajtmh.1997.57.512. View

17.

Sayin F, Dyncer S, Karaer Z, Dumanly N, Cakmak A, Incy A . Status of tick infestation of sheep and goats in Turkey. Parassitologia. 1997; 39(2):145-52. View

18.

Burt F, Leman P, Smith J, Swanepoel R . The use of a reverse transcription-polymerase chain reaction for the detection of viral nucleic acid in the diagnosis of Crimean-Congo haemorrhagic fever. J Virol Methods. 1998; 70(2):129-37. DOI: 10.1016/s0166-0934(97)00182-1. View

19.

Whitehouse C . Crimean-Congo hemorrhagic fever. Antiviral Res. 2004; 64(3):145-60. DOI: 10.1016/j.antiviral.2004.08.001. View

20.

Papa A, Papadimitriou E, Bozovic B, Antoniadis A . Genetic characterization of the M RNA segment of a Balkan Crimean-Congo hemorrhagic fever virus strain. J Med Virol. 2005; 75(3):466-9. DOI: 10.1002/jmv.20290. View