Insights into Transcription Enhancer Factor 1 (TEF-1) Activity from the Solution Structure of the TEA Domain

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 Nov 7

PMID 17085591

Citations 77

Authors

Asokan Anbanandam

Diana C Albarado

Catherine T Nguyen

Georg Halder

Xiaolian Gao

Sudha Veeraraghavan

Affiliations

Soon will be listed here.

Abstract

Transcription enhancer factor 1 is essential for cardiac, skeletal, and smooth muscle development and uses its N-terminal TEA domain (TEAD) to bind M-CAT elements. Here, we present the first structure of TEAD and show that it is a three-helix bundle with a homeodomain fold. Structural data reveal how TEAD binds DNA. Using structure-function correlations, we find that the L1 loop is essential for cooperative loading of TEAD molecules on to tandemly duplicated M-CAT sites. Furthermore, using a microarray chip-based assay, we establish that known binding sites of the full-length protein are only a subset of DNA elements recognized by TEAD. Our results provide a model for understanding the regulation of genome-wide gene expression during development by TEA/ATTS family of transcription factors.

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