Estimate of the Chloride Concentration in a Central Glutamatergic Terminal: a Gramicidin Perforated-patch Study on the Calyx of Held

Overview

Journal J Neurosci

Specialty Neurology

Date 2006 Nov 3

PMID 17079672

Citations 68

Authors

Gareth D Price

Laurence O Trussell

Affiliations

Soon will be listed here.

Abstract

The function of presynaptic terminals is regulated by intracellular Cl-, the levels of which modify vesicular endocytosis and transmitter refilling and mediate the effects of presynaptic ligand-gated Cl- channels. Nevertheless, the concentration of Cl- in a central nerve terminal is unknown, and it is unclear whether terminals can regulate Cl- independently of the soma. Using perforated-patch recording in a mammalian synapse, we found that terminals accumulate Cl- up to 21 mm, between four and five times higher than in their parent cell bodies. Changing [Cl-] did not alter vesicular glutamate content in intact terminals, unlike in vitro experiments. Thus, glutamatergic terminals maintain an elevated Cl- concentration without compromising synaptic transmission.

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