DNA Methylation Profiling of Human Chromosomes 6, 20 and 22

Overview

Journal Nat Genet

Specialty Genetics

Date 2006 Oct 31

PMID 17072317

Citations 603

Authors

Florian Eckhardt

Joern Lewin

Rene Cortese

Vardhman K Rakyan

John Attwood

Matthias Burger

John Burton

Tony V Cox

Rob Davies

Thomas A Down

Carolina Haefliger

Roger Horton

Kevin Howe

David K Jackson

Jan Kunde

Christoph Koenig

Jennifer Liddle

David Niblett

Thomas Otto

Roger Pettett

Stefanie Seemann

Christian Thompson

Tony West

Jane Rogers

Alex Olek

Kurt Berlin

Stephan Beck

Affiliations

Soon will be listed here.

Abstract

DNA methylation is the most stable type of epigenetic modification modulating the transcriptional plasticity of mammalian genomes. Using bisulfite DNA sequencing, we report high-resolution methylation profiles of human chromosomes 6, 20 and 22, providing a resource of about 1.9 million CpG methylation values derived from 12 different tissues. Analysis of six annotation categories showed that evolutionarily conserved regions are the predominant sites for differential DNA methylation and that a core region surrounding the transcriptional start site is an informative surrogate for promoter methylation. We find that 17% of the 873 analyzed genes are differentially methylated in their 5' UTRs and that about one-third of the differentially methylated 5' UTRs are inversely correlated with transcription. Despite the fact that our study controlled for factors reported to affect DNA methylation such as sex and age, we did not find any significant attributable effects. Our data suggest DNA methylation to be ontogenetically more stable than previously thought.

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