Bifidobacterium Infantis Strains with and Without a Combination of Oligofructose and Inulin (OFI) Attenuate Inflammation in DSS-induced Colitis in Rats

Overview

Journal BMC Gastroenterol

Publisher Biomed Central

Specialty Gastroenterology

Date 2006 Oct 31

PMID 17069659

Citations 42

Authors

Nadia Osman

Diya Adawi

Goran Molin

Siv Ahrne

Anna Berggren

Bengt Jeppsson

Affiliations

Soon will be listed here.

Abstract

Background: Pathogenesis of inflammatory bowel disease is thought to be through different factors and there is a relationship between the gut flora and the risk of its development. Probiotics can manipulate the microflora in chronic inflammation and may be effective in treating inflammation. Bifidobacterium are saccharolytic and their growth in the gut can be promoted by non-absorbable carbohydrates and its increase in the colon appears to be of benefit.

Methods: Oligofructose and inulin (OFI) alone and the two B. infantis DSM 15158 and DSM 15159 with and without OFI, were fed to Sprague-Dawley rats for 7 days prior to colitis induction and administrations continued for another 7 days with the DSS. Colitis severity assessed using a Disease Activity Index. Samples were collected 7 days after colitis induction, for intestinal bacterial flora, bacterial translocation, short chain fatty acids (SCFAs), myeloperoxidase (MPO), cytokines (IL-1beta, TNF-alpha, IL-10 and TGF-beta) and malondialdehyde (MDA).

Results: OFI alone or the B. infantis strains with and without OFI improved significantly the DAI and decreased colonic MPO activity. Colonic tissue IL-1beta decreased significantly in all treated groups except B. infantis DSM 15158. MDA decreased significantly in B. infantis DSM 15159 with and without OFI compared to colitis control. Succinic acid increased significantly in OFI group with and without DSM 15159 compared to all groups. Sum values of propionic, succinic acid and butyric acid increased significantly in all groups compare to the colitis control. Bacterial translocation to mesenteric lymph nodes decreased significantly in all groups compared to colitis control. Translocation to the liver decreased significantly in all groups compare to the colitis control and OFI + B. infantis DSM 15158 groups.

Conclusion: Administrations of OFI and Bifidobacterium improve DSS-induced acute colitis and have an anti-inflammatory effect. Major differences in effect were observed between the two B. infantis strains as indicated in MDA and succinic acid concentration as well as bacterial translocation rate in synbiotic combinations.

Citing Articles

Bifidogenic Effect of Human Milk Oligosaccharides on Pediatric IBD Fecal Microbiota.

Otaru N, Bajic D, Van den Abbeele P, Velde S, Van Biervliet S, Steinert R Microorganisms. 2024; 12(10).

PMID: 39458287 PMC: 11509818. DOI: 10.3390/microorganisms12101977.

FGL2 improves experimental colitis related to gut microbiota structure and bile acid metabolism by regulating macrophage autophagy and apoptosis.

Zhao Y, Xiang Z, Pan H, Huang X, Chen W, Huang Z Heliyon. 2024; 10(14):e34349.

PMID: 39104498 PMC: 11298944. DOI: 10.1016/j.heliyon.2024.e34349.

The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties.

Maftei N, Raileanu C, Balta A, Ambrose L, Boev M, Marin D Microorganisms. 2024; 12(2).

PMID: 38399637 PMC: 10891645. DOI: 10.3390/microorganisms12020234.

Mucosal host-microbe interactions associate with clinical phenotypes in inflammatory bowel disease.

Hu S, Bourgonje A, Gacesa R, Jansen B, Bjork J, Bangma A Nat Commun. 2024; 15(1):1470.

PMID: 38368394 PMC: 10874382. DOI: 10.1038/s41467-024-45855-2.

HY7718 Improves Intestinal Integrity in a DSS-Induced Ulcerative Colitis Mouse Model by Suppressing Inflammation through Modulation of the Gut Microbiota.

Kim H, Jeon H, Kim J, Shim J, Lee J Int J Mol Sci. 2024; 25(1).

PMID: 38203747 PMC: 10779067. DOI: 10.3390/ijms25010575.

References

Arai Y, Takanashi H, Kitagawa H, Okayasu I . Involvement of interleukin-1 in the development of ulcerative colitis induced by dextran sulfate sodium in mice. Cytokine. 1999; 10(11):890-6. DOI: 10.1006/cyto.1998.0355. View

Furrie E, Macfarlane S, Kennedy A, Cummings J, Walsh S, ONeil D . Synbiotic therapy (Bifidobacterium longum/Synergy 1) initiates resolution of inflammation in patients with active ulcerative colitis: a randomised controlled pilot trial. Gut. 2005; 54(2):242-9. PMC: 1774839. DOI: 10.1136/gut.2004.044834. View

Madsen K, Doyle J, Jewell L, Tavernini M, Fedorak R . Lactobacillus species prevents colitis in interleukin 10 gene-deficient mice. Gastroenterology. 1999; 116(5):1107-14. DOI: 10.1016/s0016-5085(99)70013-2. View

Gasche C, Bakos S, Dejaco C, Tillinger W, Zakeri S, Reinisch W . IL-10 secretion and sensitivity in normal human intestine and inflammatory bowel disease. J Clin Immunol. 2000; 20(5):362-70. DOI: 10.1023/a:1006672114184. View

Rada V, Petr J . A new selective medium for the isolation of glucose non-fermenting bifidobacteria from hen caeca. J Microbiol Methods. 2000; 43(2):127-32. DOI: 10.1016/s0167-7012(00)00205-0. View

Videla S, Vilaseca J, Antolin M, Garcia-Lafuente A, Guarner F, Crespo E . Dietary inulin improves distal colitis induced by dextran sodium sulfate in the rat. Am J Gastroenterol. 2001; 96(5):1486-93. DOI: 10.1111/j.1572-0241.2001.03802.x. View

Madsen K, Cornish A, Soper P, McKaigney C, Jijon H, Yachimec C . Probiotic bacteria enhance murine and human intestinal epithelial barrier function. Gastroenterology. 2001; 121(3):580-91. DOI: 10.1053/gast.2001.27224. View

Strober W, Fuss I, Blumberg R . The immunology of mucosal models of inflammation. Annu Rev Immunol. 2002; 20:495-549. DOI: 10.1146/annurev.immunol.20.100301.064816. View

Lammers K, Helwig U, Swennen E, Rizzello F, Venturi A, Caramelli E . Effect of probiotic strains on interleukin 8 production by HT29/19A cells. Am J Gastroenterol. 2002; 97(5):1182-6. DOI: 10.1111/j.1572-0241.2002.05693.x. View

10.

Borruel N, Carol M, Casellas F, Antolin M, DE LARA F, Espin E . Increased mucosal tumour necrosis factor alpha production in Crohn's disease can be downregulated ex vivo by probiotic bacteria. Gut. 2002; 51(5):659-64. PMC: 1773447. DOI: 10.1136/gut.51.5.659. View

11.

Kruidenier L, Verspaget H . Review article: oxidative stress as a pathogenic factor in inflammatory bowel disease--radicals or ridiculous?. Aliment Pharmacol Ther. 2002; 16(12):1997-2015. DOI: 10.1046/j.1365-2036.2002.01378.x. View

12.

Araki Y, Andoh A, Fujiyama Y . The free radical scavenger edaravone suppresses experimental dextran sulfate sodium-induced colitis in rats. Int J Mol Med. 2003; 12(1):125-9. View

13.

McCarthy J, OMahony L, OCallaghan L, Sheil B, Vaughan E, Fitzsimons N . Double blind, placebo controlled trial of two probiotic strains in interleukin 10 knockout mice and mechanistic link with cytokine balance. Gut. 2003; 52(7):975-80. PMC: 1773705. DOI: 10.1136/gut.52.7.975. View

14.

Cavaglieri C, Nishiyama A, Fernandes L, Curi R, Miles E, Calder P . Differential effects of short-chain fatty acids on proliferation and production of pro- and anti-inflammatory cytokines by cultured lymphocytes. Life Sci. 2003; 73(13):1683-90. DOI: 10.1016/s0024-3205(03)00490-9. View

15.

Kruidenier L, Kuiper I, Lamers C, Verspaget H . Intestinal oxidative damage in inflammatory bowel disease: semi-quantification, localization, and association with mucosal antioxidants. J Pathol. 2003; 201(1):28-36. DOI: 10.1002/path.1409. View

16.

Guarner F, Malagelada J . Role of bacteria in experimental colitis. Best Pract Res Clin Gastroenterol. 2003; 17(5):793-804. DOI: 10.1016/s1521-6918(03)00068-4. View

17.

Osman N, Adawi D, Ahrne S, Jeppsson B, Molin G . Modulation of the effect of dextran sulfate sodium-induced acute colitis by the administration of different probiotic strains of Lactobacillus and Bifidobacterium. Dig Dis Sci. 2004; 49(2):320-7. DOI: 10.1023/b:ddas.0000017459.59088.43. View

18.

Forrest C, Gould S, Darlington L, Stone T . Levels of purine, kynurenine and lipid peroxidation products in patients with inflammatory bowel disease. Adv Exp Med Biol. 2004; 527:395-400. DOI: 10.1007/978-1-4615-0135-0_46. View

19.

Segui J, Gironella M, Sans M, Granell S, Gil F, Gimeno M . Superoxide dismutase ameliorates TNBS-induced colitis by reducing oxidative stress, adhesion molecule expression, and leukocyte recruitment into the inflamed intestine. J Leukoc Biol. 2004; 76(3):537-44. DOI: 10.1189/jlb.0304196. View

20.

Lindsay J, Sandison A, Cohen P, Brennan F, Hodgson H . IL-10 gene therapy is therapeutic for dextran sodium sulfate-induced murine colitis. Dig Dis Sci. 2004; 49(7-8):1327-34. DOI: 10.1023/b:ddas.0000037830.22065.71. View